BMRB Entry 7270
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7270
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Title: 1H, 13C, 15N sequence-specific backbone and sidechain resonance assignments for a putative protein-protein interaction module from a family 84 glycoside hydrolase of Clostridium perfringens PubMed: 19636812
Deposition date: 2006-08-18 Original release date: 2007-06-27
Authors: Chitayat, Seth; Ficko-Blean, Elizabeth; Adams, Jarrett; Gregg, Katie; Boraston, Alisdair; Smith, Steven
Citation: Chitayat, Seth; Ficko-Blean, Elizabeth; Adams, Jarrett; Gregg, Katie; Boraston, Alisdair; Smith, Steven. "NMR assignment of backbone and side chain resonances for a putative protein-protein interaction module from a family 84 glycoside hydrolase of Clostridium perfringens" Biomol. NMR Assignments 1, 7-9 (2007).
Assembly members:
x82, polymer, 165 residues, Formula weight is not available
Natural source: Common Name: Clostridium Perfringens Taxonomy ID: 1502 Superkingdom: Eubacteria Kingdom: Not applicable Genus/species: Clostridium Perfringens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
x82: MGSSHHHHHHSSGLVPRGSH
MASKLKEAAEVTGSVSLEAL
EEVQVGENLEVGVGIDELVN
AEAFAYDFTLNYDENAFEYV
EAISDDGVFVNAKKIEDGKV
RVLVSSLTGEPLPAKEVLAK
VVLRAEAKAEGSNLSVTNSS
VGDGEGLVHEIAGTEKTVNI
IEGTS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 566 |
| 15N chemical shifts | 156 |
| 1H chemical shifts | 968 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | scaffold protein x82 | 1 |
Entities:
Entity 1, scaffold protein x82 165 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ALA | SER | LYS | LEU | LYS | GLU | ALA | ALA | GLU | ||||
| 4 | VAL | THR | GLY | SER | VAL | SER | LEU | GLU | ALA | LEU | ||||
| 5 | GLU | GLU | VAL | GLN | VAL | GLY | GLU | ASN | LEU | GLU | ||||
| 6 | VAL | GLY | VAL | GLY | ILE | ASP | GLU | LEU | VAL | ASN | ||||
| 7 | ALA | GLU | ALA | PHE | ALA | TYR | ASP | PHE | THR | LEU | ||||
| 8 | ASN | TYR | ASP | GLU | ASN | ALA | PHE | GLU | TYR | VAL | ||||
| 9 | GLU | ALA | ILE | SER | ASP | ASP | GLY | VAL | PHE | VAL | ||||
| 10 | ASN | ALA | LYS | LYS | ILE | GLU | ASP | GLY | LYS | VAL | ||||
| 11 | ARG | VAL | LEU | VAL | SER | SER | LEU | THR | GLY | GLU | ||||
| 12 | PRO | LEU | PRO | ALA | LYS | GLU | VAL | LEU | ALA | LYS | ||||
| 13 | VAL | VAL | LEU | ARG | ALA | GLU | ALA | LYS | ALA | GLU | ||||
| 14 | GLY | SER | ASN | LEU | SER | VAL | THR | ASN | SER | SER | ||||
| 15 | VAL | GLY | ASP | GLY | GLU | GLY | LEU | VAL | HIS | GLU | ||||
| 16 | ILE | ALA | GLY | THR | GLU | LYS | THR | VAL | ASN | ILE | ||||
| 17 | ILE | GLU | GLY | THR | SER |
Samples:
sample_1: x82, [U-13C; U-15N], 1 mM
conditions_1: pH: 7.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D CBCACONH | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 3D HC(CO)NH | not available | not available | not available |
| 3D C(CO)NH | not available | not available | not available |
| 3D aliphatic C13-NOESY-HSQC | not available | not available | not available |
| 3D N15-NOESY-HSQC | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB80940 |
| GB | ABG84519 EDT24402 EDT27538 EDT72983 EDT78323 |
| REF | NP_562150 WP_003456570 WP_003467250 WP_003471799 WP_003473459 |
| SP | Q0TR53 Q8XL08 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts