BMRB Entry 7075
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7075
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Title: Assignment of conserved hypothetical protein pa2412
Deposition date: 2006-04-17 Original release date: 2006-10-20
Authors: Lemak, Alexander; Yee, Adelinda; Lukin, Jonathan; Semesi, Anthony; Arrowsmith, Cheryl
Citation: Lemak, Alexander; Lukin, Jonathan; Yee, Adelinda; Semesi, Anthony; Arrowsmith, Cheryl. "Solution structure of pa2412 protein determined using ABACUS protocol" The BMRB entry is the only known published source for the data..
Assembly members:
conserved hypothetical protein pa2412, polymer, 72 residues, 8443 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 15597608 Superkingdom: Bacteria Kingdom: not available Genus/species: pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
conserved hypothetical protein pa2412: MTSVFDRDDIQFQVVVNHEE
QYSIWPEYKEIPQGWRAAGK
SGLKKDCLAYIEEVWTDMRP
LSLRQHMDKAAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 285 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 459 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pa2412 | 1 |
Entities:
Entity 1, pa2412 72 residues - 8443 Da.
| 1 | MET | THR | SER | VAL | PHE | ASP | ARG | ASP | ASP | ILE | ||||
| 2 | GLN | PHE | GLN | VAL | VAL | VAL | ASN | HIS | GLU | GLU | ||||
| 3 | GLN | TYR | SER | ILE | TRP | PRO | GLU | TYR | LYS | GLU | ||||
| 4 | ILE | PRO | GLN | GLY | TRP | ARG | ALA | ALA | GLY | LYS | ||||
| 5 | SER | GLY | LEU | LYS | LYS | ASP | CYS | LEU | ALA | TYR | ||||
| 6 | ILE | GLU | GLU | VAL | TRP | THR | ASP | MET | ARG | PRO | ||||
| 7 | LEU | SER | LEU | ARG | GLN | HIS | MET | ASP | LYS | ALA | ||||
| 8 | ALA | GLY |
Samples:
sample_1: conserved hypothetical protein pa2412, [U-13C; U-15N], 0.6 mM; NaCl 450 mM; MOPS 10 mM
cond_set1: ionic strength: 0.45 M; pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCAcoNH | not available | not available | not available |
| HBHAcoNH | not available | not available | not available |
| CCcoNH TOCSY | not available | not available | not available |
| HCCcoNH TOCSY | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HC(C)H TOCSY | not available | not available | not available |
| HCC(H) TOCSY | not available | not available | not available |
| 15N NOESY | not available | not available | not available |
| 13C NOESY | not available | not available | not available |
Software:
SPARKY v3.106 - assignemt, spectral analysis
NMR spectrometers:
- Bruker AVANCE 600 MHz
- Bruker AVANCE 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAK90265 BAP23198 BAP50810 GAA18000 GAJ51291 |
| EMBL | CAW27611 CCQ87246 CDH74417 CDH77199 CDI90691 |
| GB | AAG05800 AAT50634 ABJ11588 ABR86930 AEO75113 |
| REF | NP_251102 WP_003089636 WP_023084226 WP_033996385 WP_034025318 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts