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Biological Magnetic Resonance Data Bank


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BMRB Entry 6860

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6860
MolProbity Validation Chart

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Title: Chemical shift assignments of the human ortholog of tRNA endonuclease subunt SEN15   PubMed: 17166513

Deposition date: 2005-10-10 Original release date: 2005-10-20

Authors: Song, Jikui; Markley, John

Citation: Song, Jikui; Markley, John. "Three-dimensional structure determined for a subunit of human tRNA splicing endonuclease (Sen15) reveals a novel dimeric fold"  J. Mol. Biol. 366, 155-164 (2007).

Assembly members:
BC022030, polymer, 123 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
BC022030: SEDAWMGTHPKYLEMMELDI GDATQVYVAFLVYLDLMESK SWHEVNCVGLPELQLICLVG TEIEGEGLQTVVPTPITASL SHNRIREILKASRKLQGDPD LPMSFTLAIVESDSTIVYYK LTD

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts124
1H chemical shifts800

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BC0220301

Entities:

Entity 1, BC022030 123 residues - Formula weight is not available

1   SERGLUASPALATRPMETGLYTHRHISPRO
2   LYSTYRLEUGLUMETMETGLULEUASPILE
3   GLYASPALATHRGLNVALTYRVALALAPHE
4   LEUVALTYRLEUASPLEUMETGLUSERLYS
5   SERTRPHISGLUVALASNCYSVALGLYLEU
6   PROGLULEUGLNLEUILECYSLEUVALGLY
7   THRGLUILEGLUGLYGLUGLYLEUGLNTHR
8   VALVALPROTHRPROILETHRALASERLEU
9   SERHISASNARGILEARGGLUILELEULYS
10   ALASERARGLYSLEUGLNGLYASPPROASP
11   LEUPROMETSERPHETHRLEUALAILEVAL
12   GLUSERASPSERTHRILEVALTYRTYRLYS
13   LEUTHRASP

Samples:

sample_1: BC022030, [U-15N; U-13C], 0.5 ± 0.1 mM; Bis-Tris 10 ± 0.5 mM; DTT 10 ± 0.5 mM; NaCl 100 ± 5 mM

sample_2: BC022030, [U-15N; U-13C], 0.5 ± 0.1 mM; BC022030 0.5 ± 0.1 mM; Bis-Tris 10 ± 0.5 mM; DTT 10 ± 0.5 mM; NaCl 100 ± 5 mM

conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H,15N-HSQCnot availablenot availableconditions_1
1H,13C-HSQCnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HCCHTOCSYnot availablenot availableconditions_1
CCONHnot availablenot availableconditions_1
HCCONHnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
N15 T1not availablenot availableconditions_1
N15 T2not availablenot availableconditions_1
3D 15N NOESYnot availablenot availableconditions_1
3D 13C NOESYnot availablenot availableconditions_1
C13 filtered/edited NOESYnot availablenot availableconditions_1
HBCBCGCDCEHEnot availablenot availableconditions_1
HBCBCGCDHDnot availablenot availableconditions_1
HBACONHnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

PDB
DBJ BAG53590 BAG59252
GB AAG60614 AAH22030 ADQ33166 AIC52899 EAW91173
REF NP_001120866 NP_001287693 NP_001287695 NP_443197 XP_001109012
SP Q8WW01

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts