BMRB Entry 6780
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6780
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Title: NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae PubMed: 16341755
Deposition date: 2005-08-17 Original release date: 2005-12-20
Authors: Sari, Nese; Doseeva, Victoria; Subarian, Karen; Herzberg, Osnat; Orban, John
Citation: Sari, Nese; Yeh, Deok; Doseeva, Victoria; Surabian, Karen; Herzberg, Osnat; Orban, John. "NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae" J. Biomol. NMR 33, 281-281 (2005).
Assembly members:
hi1506, polymer, 131 residues, Formula weight is not available
Natural source: Common Name: Haemophilus influenza Taxonomy ID: 727 Superkingdom: Bacteria Kingdom: not available Genus/species: Haemophilus influenzae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
hi1506: GSHMDKTFCVVVQNRIKEGY
RRAGFSFHLGDNSLAAVSES
QLAQLKADPRLVVQITETGS
QEGGEGLSKEPAGSDEQKQL
RADPPSTDLNTFTVEQLKAQ
LTERGITFKQSATKAELIAL
FAPADGEKSEA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 520 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 790 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hi1506 | 1 |
Entities:
Entity 1, hi1506 131 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | ASP | LYS | THR | PHE | CYS | VAL | ||||
| 2 | VAL | VAL | GLN | ASN | ARG | ILE | LYS | GLU | GLY | TYR | ||||
| 3 | ARG | ARG | ALA | GLY | PHE | SER | PHE | HIS | LEU | GLY | ||||
| 4 | ASP | ASN | SER | LEU | ALA | ALA | VAL | SER | GLU | SER | ||||
| 5 | GLN | LEU | ALA | GLN | LEU | LYS | ALA | ASP | PRO | ARG | ||||
| 6 | LEU | VAL | VAL | GLN | ILE | THR | GLU | THR | GLY | SER | ||||
| 7 | GLN | GLU | GLY | GLY | GLU | GLY | LEU | SER | LYS | GLU | ||||
| 8 | PRO | ALA | GLY | SER | ASP | GLU | GLN | LYS | GLN | LEU | ||||
| 9 | ARG | ALA | ASP | PRO | PRO | SER | THR | ASP | LEU | ASN | ||||
| 10 | THR | PHE | THR | VAL | GLU | GLN | LEU | LYS | ALA | GLN | ||||
| 11 | LEU | THR | GLU | ARG | GLY | ILE | THR | PHE | LYS | GLN | ||||
| 12 | SER | ALA | THR | LYS | ALA | GLU | LEU | ILE | ALA | LEU | ||||
| 13 | PHE | ALA | PRO | ALA | ASP | GLY | GLU | LYS | SER | GLU | ||||
| 14 | ALA |
Samples:
sample_1: hi1506, [U-13C; U-15N], 1.0 ± 0.1 mM; potassium phosphate 50 mM; NaCl 100 mM; DTT 1 mM; D2O 90%; H2O 10%
conditions_1: ionic strength: 100 mM; pH: 7; pressure: 760 mmHg; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15n-hsqc | sample_1 | not available | conditions_1 |
| 13c-hsqc | sample_1 | not available | conditions_1 |
| hbcbhd | sample_1 | not available | conditions_1 |
| hbcbhdhe | sample_1 | not available | conditions_1 |
| hncacb | sample_1 | not available | conditions_1 |
| cbcaconh | sample_1 | not available | conditions_1 |
| hnco | sample_1 | not available | conditions_1 |
| hbhaconh | sample_1 | not available | conditions_1 |
| hcconh | sample_1 | not available | conditions_1 |
| cconh | sample_1 | not available | conditions_1 |
| 15n-noesy-hsqc | sample_1 | not available | conditions_1 |
| 13c-noesy-hsqc | sample_1 | not available | conditions_1 |
Software:
SPARKY v2.0 - manual peak assignments
NMR spectrometers:
- Bruker AVANCE 600 MHz
Download simulated HSQC data in one of the following formats:
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