BMRB Entry 6633
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6633
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Title: Structure of the RNA signal essential for translational frameshifting in HIV-1 PubMed: 15907937
Deposition date: 2005-05-13 Original release date: 2005-08-18
Authors: Gaudin, C.; Mazauric, M.; Traikia, M.; Guittet, E.; Yoshizawa, S.; Fourmy, D.
Citation: Gaudin, C.; Mazauric, M.; Traikia, M.; Guittet, E.; Yoshizawa, S.; Fourmy, D.. "Structure of the RNA Signal Essential for Translational Frameshifting in HIV-1" J. Mol. Biol. 349, 1024-1035 (2005).
Assembly members:
HIV frameshift RNA signal, polymer, 41 residues, Formula weight is not available
Natural source: Common Name: Lentivirus Taxonomy ID: 11646 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HIV frameshift RNA signal: GGCGAUCUGGCCUUCCUACA
AGGGAAGGCCAGGGAAUUGC
C
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 273 |
| 15N chemical shifts | 18 |
| 1H chemical shifts | 351 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HIV-1 frameshift RNA signal | 1 |
Entities:
Entity 1, HIV-1 frameshift RNA signal 41 residues - Formula weight is not available
| 1 | G | G | C | G | A | U | C | U | G | G | ||||
| 2 | C | C | U | U | C | C | U | A | C | A | ||||
| 3 | A | G | G | G | A | A | G | G | C | C | ||||
| 4 | A | G | G | G | A | A | U | U | G | C | ||||
| 5 | C |
Samples:
sample_1: HIV frameshift RNA signal 1 mM; Na phosphate buffer 10 mM; D2O 10%; H2O 90%
sample_2: HIV frameshift RNA signal 1 mM; Na phosphate buffer 10 mM; D2O 100%
sample_3: HIV frameshift RNA signal, [U-13C; U-15N], 1 mM; Na phosphate buffer 10 mM; D2O 100%
sample_cond_1: ionic strength: 10 mM; pH: 6.4; pressure: 1 atm; temperature: 283 K
sample_cond_2: ionic strength: 10 mM; pH: 6.4; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| HP COSY | not available | not available | not available |
| 3D-HCCH-TOCSY | not available | not available | not available |
| HSQC | not available | not available | not available |
| 3D HCP | not available | not available | not available |
Software:
xwinnmr v3.1 - processing
GIFA v4.4 - data analysis
AURELIA - data analysis
SPARKY v3.110 - data analysis
DISCOVER - refinement
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz
- Varian INOVA 500 MHz