BMRB Entry 6238
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6238
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Title: Complete 1H, 15N amd 13C Chemical Shift Assignments of the murine CBP ZZ domain (a.a. 1700-1751) PubMed: 15476823
Deposition date: 2004-06-14 Original release date: 2004-11-12
Authors: Legge, Glen; Hambly, David; Trinh, Tam; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter
Citation: Legge, Glen; Martinez-Yamout, Maria; Hambly, David; Trinh, Tam; Lee, B.; Dyson, H.; Wright, Peter. "ZZ Domain of CBP: an Unusual Zinc Finger Fold in a Protein Interaction Module" J. Mol. Biol. 343, 1081-1093 (2004).
Assembly members:
ZZ, polymer, 52 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ZZ: GQDRFVYTCNECKHHVETRW
HCTVCEDYDLCINCYNTKSH
THKMVKWGLGLD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 222 |
| 1H chemical shifts | 353 |
| 15N chemical shifts | 56 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ZZ domain of CBP | 1 |
| 2 | ZINC (II) ION, 1 | 2 |
| 3 | ZINC (II) ION, 2 | 2 |
Entities:
Entity 1, ZZ domain of CBP 52 residues - Formula weight is not available
| 1 | GLY | GLN | ASP | ARG | PHE | VAL | TYR | THR | CYS | ASN | ||||
| 2 | GLU | CYS | LYS | HIS | HIS | VAL | GLU | THR | ARG | TRP | ||||
| 3 | HIS | CYS | THR | VAL | CYS | GLU | ASP | TYR | ASP | LEU | ||||
| 4 | CYS | ILE | ASN | CYS | TYR | ASN | THR | LYS | SER | HIS | ||||
| 5 | THR | HIS | LYS | MET | VAL | LYS | TRP | GLY | LEU | GLY | ||||
| 6 | LEU | ASP |
Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: ZZ, [U-13C; U-15N], 2.3 mM; Zinc Chloride 50 uM; Salt 150 mM; Tris 20 mM; DTT 2 mM; D2O 5 % v/v
Ex-cond_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | Ex-cond_1 |
| HNCA | sample_1 | not available | Ex-cond_1 |
| CCONH TOCSY | sample_1 | not available | Ex-cond_1 |
| HCCONH TOCSY | sample_1 | not available | Ex-cond_1 |
| CBCA(CO)NH | sample_1 | not available | Ex-cond_1 |
| HCCH TOCSY | sample_1 | not available | Ex-cond_1 |
| HCCH COSY | sample_1 | not available | Ex-cond_1 |
| HNHB | sample_1 | not available | Ex-cond_1 |
| CN NOESY | sample_1 | not available | Ex-cond_1 |
| 1H/15N NOESY HSQC | sample_1 | not available | Ex-cond_1 |
| 1H13C HSQC | sample_1 | not available | Ex-cond_1 |
| 1H13C HSQC aromatic | sample_1 | not available | Ex-cond_1 |
| CGCO | sample_1 | not available | Ex-cond_1 |
| CGCN | sample_1 | not available | Ex-cond_1 |
| CBCGCD | sample_1 | not available | Ex-cond_1 |
| HNHA | sample_1 | not available | Ex-cond_1 |
| 1H15H TOSCY HSQC | sample_1 | not available | Ex-cond_1 |
Software:
NMRPIPE, Ad Bax -
NMRVIEW v5.04, One Moon Scientific -
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE06125 BAI45616 |
| GB | AAB28651 AAC17736 AAC51331 AAC51770 AAI72737 |
| PRF | 1923401A |
| REF | NP_001020603 NP_001073315 NP_001157494 NP_001247644 NP_004371 |
| SP | P45481 Q6JHU9 Q92793 |
| TPG | DAA15549 |
Download simulated HSQC data in one of the following formats:
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