BMRB Entry 6216
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6216
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Title: Solution structure of the third zinc finger domain of FOG-1 PubMed: 15234987
Deposition date: 2004-05-28 Original release date: 2005-02-08
Authors: Simpson, R.; Lee, S.; Bartle, N.; Matthews, J.; Mackay, J.; Crossley, M.
Citation: Simpson, R.; Lee, S.; Bartle, N.; Sum, E.; Visvader, J.; Matthews, J.; Mackay, J.; Crossley, M.. "A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3" J. Biol. Chem. 279, 39789-39797 (2004).
Assembly members:
ZFPM1, polymer, 35 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ZFPM1: GSSGKRPFVCRICLSAFTTK
ANCARHLKVHTDTLS
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 220 |
| coupling constants | 31 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Zinc finger protein ZFPM1 | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, Zinc finger protein ZFPM1 35 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | LYS | ARG | PRO | PHE | VAL | CYS | ||||
| 2 | ARG | ILE | CYS | LEU | SER | ALA | PHE | THR | THR | LYS | ||||
| 3 | ALA | ASN | CYS | ALA | ARG | HIS | LEU | LYS | VAL | HIS | ||||
| 4 | THR | ASP | THR | LEU | SER |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: ZFPM1 0.27 mM; ZnSO4 1 mM; TCEP 2 mM; H2O 95%; D2O 5%
sample_2: ZFPM1, [U-15N], 0.245 mM; ZnSO4 1 mM; TCEP 2 mM; H2O 95%; D2O 5%
sample_cond_1: ionic strength: 0 mM; pH: 5.6; pressure: 1 atm; temperature: 275 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
Software:
xwinnmr v2.6 - processing
XEASY v1.3.13 - data analysis
DYANA v1.5 - refinement
ARIA v1.2 - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |