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BMRB Entry 6043

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6043
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Title: 1H, 13C and 15N resonance assignment of Cu(I)-Pseudoazurin from Alcaligenes faecalis S-6   PubMed: 15243189

Deposition date: 2003-12-12 Original release date: 2004-07-29

Authors: Impagliazzo, Antonietta; Ubbink, Marcellus

Citation: Impagliazzo, Antonietta; Ubbink, Marcellus. "Letter to the Editor: 1H, 13C and 15N resonance assignment of Cu(I)-Pseudoazurin from Alcaligenes faecalis S-6 "  J. Biomol. NMR 29, 541-542 (2004).

Assembly members:
Pseudoazurin, polymer, 125 residues, 13524.69 Da.
CU1, non-polymer, 63.546 Da.

Natural source:   Common Name: Alcaligenes faecalis   Taxonomy ID: 511   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Alcaligenes faecalis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Pseudoazurin: ASENIEVHMLNKGAEGAMVF EPAYIKANPGDTVTFIPVDK GHNVESIKDMIPEGAEKFKS KINENYVLTVTQPGAYLVKC TPHYAMGMIALIAVGDSPAN LDQIVSAKKPKIVQERLEKV IASAK

Data sets:
Data typeCount
15N chemical shifts124
1H chemical shifts806
13C chemical shifts497

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1pseudoazurin1
2COPPER (I) ION2

Entities:

Entity 1, pseudoazurin 125 residues - 13524.69 Da.

1   ALASERGLUASNILEGLUVALHISMETLEU
2   ASNLYSGLYALAGLUGLYALAMETVALPHE
3   GLUPROALATYRILELYSALAASNPROGLY
4   ASPTHRVALTHRPHEILEPROVALASPLYS
5   GLYHISASNVALGLUSERILELYSASPMET
6   ILEPROGLUGLYALAGLULYSPHELYSSER
7   LYSILEASNGLUASNTYRVALLEUTHRVAL
8   THRGLNPROGLYALATYRLEUVALLYSCYS
9   THRPROHISTYRALAMETGLYMETILEALA
10   LEUILEALAVALGLYASPSERPROALAASN
11   LEUASPGLNILEVALSERALALYSLYSPRO
12   LYSILEVALGLNGLUARGLEUGLULYSVAL
13   ILEALASERALALYS

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: Pseudoazurin, [U-95% 13C; U-98% 15N], 2.0 mM; K-Phosphate buffer 20 mM; ascorbate 2 mM; D2O 10%

sample_2: Pseudoazurin, [U-98% 15N], 3.0 mM; K-Phosphate buffer 20 mM; ascorbate 2 mM; D2O 10%

sample_3: Pseudoazurin, [U-98% 15N], 2.0 mM; K-Phosphate buffer 20 mM; ascorbate 2 mM; D2O 100%

Ex-cond_1: pH: 7.0 na; temperature: 312 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HNCACOnot availablenot availableEx-cond_1
2D 15N-HSQC-TOCSYnot availablenot availableEx-cond_1
2D 15N-HSQC-NOESYnot availablenot availableEx-cond_1
13C-HSQCnot availablenot availableEx-cond_1
H(CCO)NHnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
13C-HSQCnot availablenot availableEx-cond_1
HCCHnot availablenot availableEx-cond_1
13C-HSQC-NOESYnot availablenot availableEx-cond_1

Software:

ANSIG vfor windows 1.0 - assignment

AZARA - processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

SWISS-PROT P04377
REF YP_001371437
PRF 1204196A
GenBank ABS15608 AAA21955
PDB

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