BMRB Entry 6030
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6030
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Title: 1H, 13C and 15N resonance assignment of the nucleotide binding domain of KdpB from Escherichia coli PubMed: 15213457
Deposition date: 2003-12-03 Original release date: 2004-07-06
Authors: Haupt, Melina; Coles, Murray; Truffault, Vincent; Bramkamp, Marc; Altendorf, Karlheinz; Kessler, Horst
Citation: Haupt, Melina; Coles, Murray; Truffault, Vincent; Bramkamp, Marc; Altendorf, Karlheinz; Kessler, Horst. "Letter to the Editor: 1H, 13C and 15N resonance assignment of the nucleotide binding domain of KdpB from Escherichia coli" J. Biomol. NMR 29, 437-438 (2004).
Assembly members:
potassium transporting ATPase, polymer, 156 residues, 17000 Da.
ANP, non-polymer, 506.196 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
potassium transporting ATPase: MGHHHHHHHHHHSSGHGGRH
NRQASEFIPAQGVDEKTLAD
AAQLASLADETPEGRSIVIL
AKQRFNLRERDVQSLHATFV
PFTAQSRMSGINIDNRMIRK
GSVDAIRRHVEANGGHFPTD
VDQKVDQVARQGATPLVVVE
GSRVLGVIALKDIVKG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 898 |
| 13C chemical shifts | 413 |
| 15N chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KdpBN | 1 |
| 2 | ANP | 2 |
Entities:
Entity 1, KdpBN 156 residues - 17000 Da.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | HIS | SER | SER | GLY | HIS | GLY | GLY | ARG | HIS | ||||
| 3 | ASN | ARG | GLN | ALA | SER | GLU | PHE | ILE | PRO | ALA | ||||
| 4 | GLN | GLY | VAL | ASP | GLU | LYS | THR | LEU | ALA | ASP | ||||
| 5 | ALA | ALA | GLN | LEU | ALA | SER | LEU | ALA | ASP | GLU | ||||
| 6 | THR | PRO | GLU | GLY | ARG | SER | ILE | VAL | ILE | LEU | ||||
| 7 | ALA | LYS | GLN | ARG | PHE | ASN | LEU | ARG | GLU | ARG | ||||
| 8 | ASP | VAL | GLN | SER | LEU | HIS | ALA | THR | PHE | VAL | ||||
| 9 | PRO | PHE | THR | ALA | GLN | SER | ARG | MET | SER | GLY | ||||
| 10 | ILE | ASN | ILE | ASP | ASN | ARG | MET | ILE | ARG | LYS | ||||
| 11 | GLY | SER | VAL | ASP | ALA | ILE | ARG | ARG | HIS | VAL | ||||
| 12 | GLU | ALA | ASN | GLY | GLY | HIS | PHE | PRO | THR | ASP | ||||
| 13 | VAL | ASP | GLN | LYS | VAL | ASP | GLN | VAL | ALA | ARG | ||||
| 14 | GLN | GLY | ALA | THR | PRO | LEU | VAL | VAL | VAL | GLU | ||||
| 15 | GLY | SER | ARG | VAL | LEU | GLY | VAL | ILE | ALA | LEU | ||||
| 16 | LYS | ASP | ILE | VAL | LYS | GLY |
Entity 2, ANP - C10 H17 N6 O12 P3 - 506.196 Da.
| 1 | ANP |
Samples:
KdpBN-ANP: Type IA potassium translocating ATPase B chain, [U-13C; U-15N], 1.0 mM; PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER0.5 – 15.0 mM
KdpBN-ANP_cond: pH: 6.0 na; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCA(CO)NH | KdpBN-ANP | not available | KdpBN-ANP_cond |
| (H)CCH-COSY | KdpBN-ANP | not available | KdpBN-ANP_cond |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz
- Bruker DMX 750 MHz
- Bruker DMX 900 MHz
Related Database Links:
| BMRB | 6029 |
| PDB | |
| DBJ | BAA35354 BAB34148 BAG76281 BAI24086 BAI29554 |
| EMBL | CAP75183 CAQ31162 CAQ89912 CAQ97541 CAR02071 |
| GB | AAB96336 AAC73791 AAG55018 AAN79255 AAZ87406 |
| REF | NP_286410 NP_308752 NP_415225 NP_752712 WP_000087925 |
| SP | A1A8W1 A7ZJ80 A7ZXV8 B1IY32 B1LLE1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts