BMRB Entry 5961
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5961
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Title: Binding site structure of one LRP/RAP complex - implications for a common ligand/receptor binding motif PubMed: 16938309
Deposition date: 2003-09-30 Original release date: 2007-03-22
Authors: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzeroth, Michael; Thoegersen, Hans; Poulsen, Flemming; Kragelund, Birthe
Citation: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzerodt, Michael; Thoegersen, Hans; O'Shea, C.; Poulsen, Flemming; Kragelund, Birthe. "Binding site structure of one LRP-RAP complex: implications for a common ligand-receptor binding motif" J. Mol. Biol. 362, 700-716 (2006).
Assembly members:
complement type repeats 5 and 6, polymer, 82 residues, 8938.61 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichis coli
Entity Sequences (FASTA):
complement type repeats 5 and 6: SARTCPPNQFSCASGRCIPI
SWTCDLDDDCGDRSDESASC
AYPTCFPLTQFTCNNGRCIN
INWRCDNDNDCGDNSDEAGC
SH
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 436 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CR56 | 1 |
| 2 | CALCIUM (II) ION, 1 | 2 |
| 3 | CALCIUM (II) ION, 2 | 2 |
Entities:
Entity 1, CR56 82 residues - 8938.61 Da.
| 1 | SER | ALA | ARG | THR | CYS | PRO | PRO | ASN | GLN | PHE | ||||
| 2 | SER | CYS | ALA | SER | GLY | ARG | CYS | ILE | PRO | ILE | ||||
| 3 | SER | TRP | THR | CYS | ASP | LEU | ASP | ASP | ASP | CYS | ||||
| 4 | GLY | ASP | ARG | SER | ASP | GLU | SER | ALA | SER | CYS | ||||
| 5 | ALA | TYR | PRO | THR | CYS | PHE | PRO | LEU | THR | GLN | ||||
| 6 | PHE | THR | CYS | ASN | ASN | GLY | ARG | CYS | ILE | ASN | ||||
| 7 | ILE | ASN | TRP | ARG | CYS | ASP | ASN | ASP | ASN | ASP | ||||
| 8 | CYS | GLY | ASP | ASN | SER | ASP | GLU | ALA | GLY | CYS | ||||
| 9 | SER | HIS |
Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: complement type repeats 5 and 6 0.5 mM; CaCl2 10 mM; MgCl2 1 mM; NaCl 100 mM
sample_2: complement type repeats 5 and 6, [U-99% 15N], 0.5 mM; CaCl2 10 mM; MgCl2 1 mM; NaCl 100 mM
conditions: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | conditions |
| NOESY | not available | not available | conditions |
| TOCSY | not available | not available | conditions |
| 15N-HSQC | not available | not available | conditions |
| 15N-NOESY-HSQC | not available | not available | conditions |
| 15N-TOCSY-HSQC | not available | not available | conditions |
Software:
PRONTO v20020517 - assignment tool
NMR spectrometers:
- Varian UNITY 750 MHz
- Varian UNITY 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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