BMRB Entry 5333
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5333
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Title: Redox-Coupled Conformational Alternations in Cytochrome c3 from D. vulgaris Miyazaki F on the Basis of its Reduced Solution Structure PubMed: 12054869
Deposition date: 2002-03-26 Original release date: 2002-06-13
Authors: Harada, Erisa; Fukuoka, Yuki; Ohmura, Tomoaki; Fukunishi, Arima; Kawai, Gota; Fujiwara, Toshimichi; Akutsu, Hideo
Citation: Harada, Erisa; Fukuoka, Yuki; Ohmura, Tomoaki; Fukunishi, Arima; Kawai, Gota; Fujiwara, Toshimichi; Akutsu, Hideo. "Redox-coupled Conformational Alternations in Cytochrome c3 from D. vulgaris Miyazaki F on the Basis of its Reduced Solution Structure" J. Mol. Biol. 319, 767-778 (2002).
Assembly members:
cytochrome c3, polymer, 107 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: not available Taxonomy ID: 883 Superkingdom: Bacteria Kingdom: not available Genus/species: Desulfovibrio vulgaris
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
cytochrome c3: APKAPADGLKMDKTKQPVVF
NHSTHKAVKCGDCHHPVNGK
EDYQKCATAGCHDNMDKKDK
SAKGYYHAMHDKGTKFKSCV
GCHLETAGADAAKKKELTGC
KGSKCHS
| Data type | Count |
| 1H chemical shifts | 782 |
| 15N chemical shifts | 115 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome c3 peptide | 1 |
| 2 | heme 1 | 2 |
| 3 | heme 2 | 2 |
| 4 | heme 3 | 2 |
| 5 | heme 4 | 2 |
Entities:
Entity 1, cytochrome c3 peptide 107 residues - Formula weight is not available
| 1 | ALA | PRO | LYS | ALA | PRO | ALA | ASP | GLY | LEU | LYS | ||||
| 2 | MET | ASP | LYS | THR | LYS | GLN | PRO | VAL | VAL | PHE | ||||
| 3 | ASN | HIS | SER | THR | HIS | LYS | ALA | VAL | LYS | CYS | ||||
| 4 | GLY | ASP | CYS | HIS | HIS | PRO | VAL | ASN | GLY | LYS | ||||
| 5 | GLU | ASP | TYR | GLN | LYS | CYS | ALA | THR | ALA | GLY | ||||
| 6 | CYS | HIS | ASP | ASN | MET | ASP | LYS | LYS | ASP | LYS | ||||
| 7 | SER | ALA | LYS | GLY | TYR | TYR | HIS | ALA | MET | HIS | ||||
| 8 | ASP | LYS | GLY | THR | LYS | PHE | LYS | SER | CYS | VAL | ||||
| 9 | GLY | CYS | HIS | LEU | GLU | THR | ALA | GLY | ALA | ASP | ||||
| 10 | ALA | ALA | LYS | LYS | LYS | GLU | LEU | THR | GLY | CYS | ||||
| 11 | LYS | GLY | SER | LYS | CYS | HIS | SER |
Entity 2, heme 1 - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Samples:
sample: cytochrome c3, [U-15N], 1.0 – 2.0 mM
sample_conditon: pH: 7.0 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | sample | not available | sample_conditon |
| 1H-15N TOCSY | sample | not available | sample_conditon |
| NOESY | sample | not available | sample_conditon |
| DQF-COSY | sample | not available | sample_conditon |
| E-COSY | sample | not available | sample_conditon |
Software:
NMR-Pipe/Pipp v4.0 -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker AMX 500 MHz
- Bruker DRX 400 MHz
Related Database Links:
| BMRB | 6572 |
| PDB | |
| DBJ | BAA06511 |
| GB | ACL09439 EGY24874 |
| PRF | 0607221A 1916446A |
| REF | WP_007526318 WP_015946129 YP_002436907 |
| SP | P00132 |
Download simulated HSQC data in one of the following formats:
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