BMRB Entry 5141
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5141
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Title: Sequence-specific resonance assignments of the N-terminal, 105-residue KaiC-interacting domain of SasA, a protein necessary for a robust circadian rhythm in Synechococcus elongatus
Deposition date: 2001-09-01 Original release date: 2003-03-12
Authors: Klewer, Douglas; Williams, Stanly; Golden, Susan; LiWang, Andy
Citation: Klewer, Douglas; Williams, Stanly; Golden, Susan; LiWang, Andy. "Letter to the Editor: Sequence-specific resonance assignments of the N-terminal, 105-residue KaiC-interacting domain of SasA, a protein necessary for a robust circadian rhythm in Synechococcus elongatus" J. Biomol. NMR 24, 77-78 (2002).
Assembly members:
SasA, polymer, 105 residues, 11476.3 Da.
Natural source: Common Name: Synechoccus elongatus Taxonomy ID: not available Superkingdom: Eubacteria Kingdom: not available Genus/species: Synechoccus elongatus
Experimental source: Production method: recombinant technology Host organism: coli
Entity Sequences (FASTA):
SasA: GSSLSPQALAQPLLLQLFVD
TRPLSQHIVQRVKNILAAVE
ATVPISLQVINVADQPQLVE
YYRLVVTPALVKIGPGSRQV
LSGIDLTDQLANQLPQWLVQ
QEGIF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 343 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 683 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SasA N-terminus | 1 |
Entities:
Entity 1, SasA N-terminus 105 residues - 11476.3 Da.
| 1 | GLY | SER | SER | LEU | SER | PRO | GLN | ALA | LEU | ALA | ||||
| 2 | GLN | PRO | LEU | LEU | LEU | GLN | LEU | PHE | VAL | ASP | ||||
| 3 | THR | ARG | PRO | LEU | SER | GLN | HIS | ILE | VAL | GLN | ||||
| 4 | ARG | VAL | LYS | ASN | ILE | LEU | ALA | ALA | VAL | GLU | ||||
| 5 | ALA | THR | VAL | PRO | ILE | SER | LEU | GLN | VAL | ILE | ||||
| 6 | ASN | VAL | ALA | ASP | GLN | PRO | GLN | LEU | VAL | GLU | ||||
| 7 | TYR | TYR | ARG | LEU | VAL | VAL | THR | PRO | ALA | LEU | ||||
| 8 | VAL | LYS | ILE | GLY | PRO | GLY | SER | ARG | GLN | VAL | ||||
| 9 | LEU | SER | GLY | ILE | ASP | LEU | THR | ASP | GLN | LEU | ||||
| 10 | ALA | ASN | GLN | LEU | PRO | GLN | TRP | LEU | VAL | GLN | ||||
| 11 | GLN | GLU | GLY | ILE | PHE |
Samples:
sample_SasA-105: SasA, [U-99% 13C; U-99% 15N], 0.6 mM
condition_1: ionic strength: 0.22 M; pH: 7.0 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCA(CO)NH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| HNHA | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| H(C)CH-COSY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
Software:
NMRPipe v1.8 rev 2001.030.21.27 - NMR data processing
PIPP v4.2.6 - Spectra visualization
STAPP v4.2.6 - partial sequence specific assignments, spin system creation
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA03145 BAD80168 |
| GB | ABB58144 AJD57380 |
| REF | WP_011244288 WP_011378322 WP_039755926 YP_172688 YP_401131 |
| SP | Q06904 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts