BMRB Entry 4995
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4995
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Title: Solution NMR Structure and Folding Dynamics of the N-terminus of a rat Non-muscle Alpha-tropomyosin in an Engineered Chimeric Protein PubMed: 11575936
Deposition date: 2001-04-20 Original release date: 2002-04-03
Authors: Greenfield, Norma; Huang, Yuanpeng; Palm, Thomas; Swapna, G.V.T.S.; Monleon, Daniel; Montelione, Gaetano; Hitchcock-DeGregori, Sarah
Citation: Greenfield, Norma; Huang, Yuanpeng; Palm, Thomas; Swapna, G.V.T.S.; Monleon, Daniel; Montelione, Gaetano; Hitchcock-DeGregori, Sarah. "Solution NMR Structure and Folding Dynamics of the N-terminus of a rat Non-muscle Alpha-tropomyosin in an Engineered Chimeric Protein" J. Mol. Biol. 312, 833-847 (2001).
Assembly members:
GlyTM1bZip, polymer, 38 residues, 8644 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
GlyTM1bZip: GAGSSSLEAVRRKIRSLQEQ
NYHLENEVARLKKLVGER
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 274 |
| 13C chemical shifts | 160 |
| 15N chemical shifts | 41 |
| coupling constants | 34 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GlyTM1bZip subunit A | 1 |
| 2 | GlyTM1bZip subunit B | 1 |
Entities:
Entity 1, GlyTM1bZip subunit A 38 residues - 8644 Da.
| 1 | GLY | ALA | GLY | SER | SER | SER | LEU | GLU | ALA | VAL | ||||
| 2 | ARG | ARG | LYS | ILE | ARG | SER | LEU | GLN | GLU | GLN | ||||
| 3 | ASN | TYR | HIS | LEU | GLU | ASN | GLU | VAL | ALA | ARG | ||||
| 4 | LEU | LYS | LYS | LEU | VAL | GLY | GLU | ARG |
Samples:
GlyTM1bZip_(two-chained): GlyTM1bZip coiled-coil dimer 0.6 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%
15N-GlyTM1bZip: GlyTM1bZip coiled-coil dimer, [U-99% 15N], 0.5 – 2.0 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%
15N_13C-GlyTM1bZip: GlyTM1bZip coiled-coil dimer, [U-99% 13C; U-99% 15N], 0.8 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%
GlyTM1bZip_heterodimer: GlyTM1bZip coiled-coil dimer 1.32 mM; GlyTM1bZip coiled-coil dimer, [U-99% 13C; U-99% 15N], 0.88 mM; NaCl 100 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%
conditions_1: pH: 6.4; temperature: 281 K; ionic strength: 0.12 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-1H HSQC | not available | not available | not available |
| 13C-!H HSQC | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| 2QF-COSY | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| CANH | not available | not available | not available |
| H(CA)NH | not available | not available | not available |
| H(CA)(CO)NH | not available | not available | not available |
| (CA)(C0)NH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CNCA(CO)NH | not available | not available | not available |
| H_HCC(CO)NH-TOCSY | not available | not available | not available |
| C_HCC(CO)NH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| 15N edited PFG NOESY | not available | not available | not available |
| 13C edited PFG NOESY | not available | not available | not available |
| 13C X-filtered NOESY | not available | not available | not available |
| 13C-15N X-filtered NOESY | not available | not available | not available |
Software:
VNMR v5.3B - Data Processing
SPARKY v3.74 - Peak Picking
AutoAssign v1.5 - Automatic Assignments
AutoStructure v1.0 - Automatic Assignments of NOESY peaks, Structure determination
Dyana v1.5 - Structure determination using torsion angle dynamics
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| BMRB | 15965 |
| PDB | |
| DBJ | BAD92278 BAG11063 BAH12832 BAH13458 |
| GB | AAA18098 AAA18099 AAA42253 AAH50473 AAH53545 |
| REF | NP_001018008 NP_001029245 NP_001029246 NP_001029247 NP_001157724 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts