BMRB Entry 4941
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4941
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70 PubMed: 11457852
Deposition date: 2001-01-17 Original release date: 2002-01-23
Authors: Zhang, Ziming; Zhu, Lingyang; Lin, Donghai; Chen, F.; Chen, David; Chen, Yuan
Citation: Zhang, Ziming; Zhu, Lingyang; Lin, Donghai; Chen, F.; Chen, David; Chen, Yuan. "The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70" J. Biol. Chem. 276, 38231-38236 (2001).
Assembly members:
Ku70, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Ku70: MGSSHHHHHHSSGLVPRGSH
MASPEGKVTKRKHDNEGSGS
KRPKVEYSEEELKTHISKGT
LGKFTVPMLKEACRAYGLKS
GLKKQELLEALTKHFQD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 288 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 600 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ku70 CTD | 1 |
Entities:
Entity 1, Ku70 CTD 97 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ALA | SER | PRO | GLU | GLY | LYS | VAL | THR | LYS | ||||
| 4 | ARG | LYS | HIS | ASP | ASN | GLU | GLY | SER | GLY | SER | ||||
| 5 | LYS | ARG | PRO | LYS | VAL | GLU | TYR | SER | GLU | GLU | ||||
| 6 | GLU | LEU | LYS | THR | HIS | ILE | SER | LYS | GLY | THR | ||||
| 7 | LEU | GLY | LYS | PHE | THR | VAL | PRO | MET | LEU | LYS | ||||
| 8 | GLU | ALA | CYS | ARG | ALA | TYR | GLY | LEU | LYS | SER | ||||
| 9 | GLY | LEU | LYS | LYS | GLN | GLU | LEU | LEU | GLU | ALA | ||||
| 10 | LEU | THR | LYS | HIS | PHE | GLN | ASP |
Samples:
sample_1: Ku70, [U-15N], 0.8 – 1.5 mM; phosphate buffer 100 mM; NaN3 0.03%; DTT 5 mM
sample_2: Ku70, [U-13C; U-15N], 0.8 – 1.5 mM; phosphate buffer 100 mM; NaN3 0.03%; DTT 5 mM
conditions_1: pH: 6.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HCCH_TOCSY | not available | not available | not available |
| 15N-edited | not available | not available | not available |
| TOCSY-HSQC | not available | not available | not available |
| DQFCOSY | not available | not available | not available |
| 13C-edited | not available | not available | not available |
| NOESY | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
Software:
FELIX v98 -
NMR spectrometers:
- Varin Unity-plus 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAE00743 BAE01402 BAG10952 BAG37986 BAG51575 |
| EMBL | CAG30378 CAG47015 CAH93208 CAK54520 CAK54819 |
| GB | AAA36155 AAA51733 AAA61177 AAB22381 AAH08343 |
| REF | NP_001126888 NP_001267434 NP_001271637 NP_001275905 NP_001275906 |
| SP | P12956 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts