BMRB Entry 4930
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4930
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Title: 1H and 15N Chemical Shift Assignments for the homodimer of human TFF1 PubMed: 11286998
Deposition date: 2000-12-22 Original release date: 2001-01-04
Authors: Williams, Mark; Westley, Bruce; May, Felicity; Feeney, James
Citation: Williams, Mark; Westley, Bruce; May, Felicity; Feeney, James. "The Solution Structure of the Disulphide-linked Homodimer of the Human Trefoil Protein TFF1" FEBS Lett. 493, 70-74 (2001).
Assembly members:
Trefoil Factor Family 1, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Trefoil Factor Family 1: EAQTETCTVAPRERQNCGFP
GVTPSQCANKGCCFDDTVRG
VPWCFYPNTIDVPPEEECEF
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 386 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
| 2 | subunit 2 | 1 |
Entities:
Entity 1, subunit 1 60 residues - Formula weight is not available
| 1 | GLU | ALA | GLN | THR | GLU | THR | CYS | THR | VAL | ALA | |
| 2 | PRO | ARG | GLU | ARG | GLN | ASN | CYS | GLY | PHE | PRO | |
| 3 | GLY | VAL | THR | PRO | SER | GLN | CYS | ALA | ASN | LYS | |
| 4 | GLY | CYS | CYS | PHE | ASP | ASP | THR | VAL | ARG | GLY | |
| 5 | VAL | PRO | TRP | CYS | PHE | TYR | PRO | ASN | THR | ILE | |
| 6 | ASP | VAL | PRO | PRO | GLU | GLU | GLU | CYS | GLU | PHE |
Samples:
sample_1: Trefoil Factor Family 1, U-95% 15N, 2 mM; sodium phosphate 5 mM
cond_1: ionic strength: 0.01 M; pH: 5.9; temperature: 298 K
cond_2: ionic strength: 0.01 M; pH: 5.9; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| ROESY | not available | not available | not available |
| 15N-1H HSQC | not available | not available | not available |
| 15N-1H HSQC-NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian UNITY 600 MHz
- Varian UNITY PLUS 500 MHz
Related Database Links:
| BMRB | 4933 |
| PDB | |
| DBJ | BAA95532 BAB13729 |
| EMBL | CAA25155 CAA28695 CAA36254 |
| GenBank | AAA52402 AAH32811 AAX36234 AAX36702 AAX41076 |
| PRF | 1502207A 1716375A |
| REF | NP_003216 |
| SWISS-PROT | P04155 |
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