BMRB Entry 4884
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4884
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Title: 1st LIM domain of PINCH protein PubMed: 11078733
Deposition date: 2000-10-25 Original release date: 2001-03-12
Authors: Velyvis, Algirdas; Yang, Yanwu; Wu, Chuanyue; Qin, Jun
Citation: Velyvis, Algirdas; Yang, Yanwu; Wu, Chuanyue; Qin, Jun. "Solution Structure of Focal Adhesion Adaptor PINCH LIM1 Domain and Characterization of Its Interaction with Integrin Linked Kinase Ankyrin Repeat Domain" J. Biol. Chem. 276, 4932-4939 (2001).
Assembly members:
1st LIM domain of PINCH protein, polymer, 77 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
1st LIM domain of PINCH protein: ISEFMANALASATCERCKGG
FAPAEKIVNSNGELYHEQCF
VCAQCFQQFPEGLFYEFEGR
KYCEHDFQMLFAPCWIL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 410 |
| 13C chemical shifts | 202 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LIM1 domain | 1 |
| 2 | Zn-1 | 2 |
| 3 | Zn-2 | 2 |
Entities:
Entity 1, LIM1 domain 77 residues - Formula weight is not available
| 1 | ILE | SER | GLU | PHE | MET | ALA | ASN | ALA | LEU | ALA | ||||
| 2 | SER | ALA | THR | CYS | GLU | ARG | CYS | LYS | GLY | GLY | ||||
| 3 | PHE | ALA | PRO | ALA | GLU | LYS | ILE | VAL | ASN | SER | ||||
| 4 | ASN | GLY | GLU | LEU | TYR | HIS | GLU | GLN | CYS | PHE | ||||
| 5 | VAL | CYS | ALA | GLN | CYS | PHE | GLN | GLN | PHE | PRO | ||||
| 6 | GLU | GLY | LEU | PHE | TYR | GLU | PHE | GLU | GLY | ARG | ||||
| 7 | LYS | TYR | CYS | GLU | HIS | ASP | PHE | GLN | MET | LEU | ||||
| 8 | PHE | ALA | PRO | CYS | TRP | ILE | LEU |
Entity 2, Zn-1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: 1st LIM domain of PINCH protein 0.5 mM; Na phosphate 50 mM; NaCl 100 mM; beta-mercaptoethanol 0.5 mM
sample_2: 1st LIM domain of PINCH protein, [U-15N], 0.5 mM; Na phosphate 50 mM; NaCl 100 mM; beta-mercaptoethanol 0.5 mM
sample_3: 1st LIM domain of PINCH protein, [U-15N; U-13C], 0.5 mM; Na phosphate 50 mM; NaCl 100 mM; beta-mercaptoethanol 0.5 mM
representative_conditions: pH: 7.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | representative_conditions |
| 3D CBCANH | not available | not available | representative_conditions |
| 3D CBCA(CO)NH | not available | not available | representative_conditions |
| 3D 1H-1H-13C/15N NOESY | not available | not available | representative_conditions |
| 3D 1H-13C-1H HCCH-TOCSY | not available | not available | representative_conditions |
| 3D C(CO)NH | not available | not available | representative_conditions |
| 3D H(CCO)NH | not available | not available | representative_conditions |
| 3D HNHA | not available | not available | representative_conditions |
| 2D 1H-1H NOESY | not available | not available | representative_conditions |
| 2D 1H-1H TOCSY | not available | not available | representative_conditions |
Software:
nmrPipe -
PIPP -
NMR spectrometers:
- Varian Unity Plus 500 MHz
Related Database Links:
| SWISS-PROT | Q9HB10 |
| REF | XP_862963 NP_277049 |
| GenBank | AAY24131 AAY14903 AAI12234 AAH93812 AAF99328 |
| DBJ | BAG60588 BAG35111 |
| PDB |
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