BMRB Entry 4407
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4407
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Title: NMR chemical shift assignment of human GAIP (Galpha Interacting Protein): A regulator of G protein signaling
Deposition date: 1999-09-15 Original release date: 1999-11-11
Authors: de Alba, E.; De Vries, L.; Farquhar, M.; Tjandra, N.
Citation: de Alba, E.; De Vries, L.; Farquhar, M.; Tjandra, N.. "Solution structure of human GAIP (G alpha interacting protein). A regulator of G protein signaling" J. Mol. Biol. 291, 927-939 (1999).
Assembly members:
Gaip protein, polymer, 153 residues, 17321 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Gaip protein: GSPGISGGGGGIPSPEEVQS
WAQSFDKLMHSPAGRSVFRA
FLRTEYSEENMLFWLACEEL
KAEANQHVVDEKARLIYEDY
VSILSPKEVSLDSRVREGIN
KKMQEPSAHTFDDAQLQIYT
LMHRDSYPRFLSSPTYRALL
PWVDSSSSLIHRD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 452 |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 899 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gaip | 1 |
Entities:
Entity 1, gaip 153 residues - 17321 Da.
| 1 | GLY | SER | PRO | GLY | ILE | SER | GLY | GLY | GLY | GLY | ||||
| 2 | GLY | ILE | PRO | SER | PRO | GLU | GLU | VAL | GLN | SER | ||||
| 3 | TRP | ALA | GLN | SER | PHE | ASP | LYS | LEU | MET | HIS | ||||
| 4 | SER | PRO | ALA | GLY | ARG | SER | VAL | PHE | ARG | ALA | ||||
| 5 | PHE | LEU | ARG | THR | GLU | TYR | SER | GLU | GLU | ASN | ||||
| 6 | MET | LEU | PHE | TRP | LEU | ALA | CYS | GLU | GLU | LEU | ||||
| 7 | LYS | ALA | GLU | ALA | ASN | GLN | HIS | VAL | VAL | ASP | ||||
| 8 | GLU | LYS | ALA | ARG | LEU | ILE | TYR | GLU | ASP | TYR | ||||
| 9 | VAL | SER | ILE | LEU | SER | PRO | LYS | GLU | VAL | SER | ||||
| 10 | LEU | ASP | SER | ARG | VAL | ARG | GLU | GLY | ILE | ASN | ||||
| 11 | LYS | LYS | MET | GLN | GLU | PRO | SER | ALA | HIS | THR | ||||
| 12 | PHE | ASP | ASP | ALA | GLN | LEU | GLN | ILE | TYR | THR | ||||
| 13 | LEU | MET | HIS | ARG | ASP | SER | TYR | PRO | ARG | PHE | ||||
| 14 | LEU | SER | SER | PRO | THR | TYR | ARG | ALA | LEU | LEU | ||||
| 15 | PRO | TRP | VAL | ASP | SER | SER | SER | SER | LEU | ILE | ||||
| 16 | HIS | ARG | ASP |
Samples:
sample_1: Gaip protein, [U-13C; U-15N], 0.6 – 1.0 mM
sample_cond_1: ionic strength: 5 mM; pH: 7.2; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCACONH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| HBHACONH | not available | not available | not available |
| HNCA | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| C-CNOESY | not available | not available | not available |
| N-CNOESY | not available | not available | not available |
| H-HNOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAG33711 |
| GB | AAI23822 EDL07441 EDL07445 EPY87810 |
| REF | NP_001070383 XP_003462829 XP_003787844 XP_005214874 XP_006045501 |
| SP | Q08DC7 |
| TPG | DAA23284 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts