BMRB Entry 4269
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4269
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Title: 1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase
Deposition date: 1998-11-18 Original release date: 1999-10-14
Authors: Pan, Borlan; Mullen, Greg
Citation: Pan, Borlan; Mullen, Greg. "1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase" J. Biomol. NMR 13, 307-308 (1999).
Assembly members:
resolvase, polymer, 105 residues, 11608 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
resolvase: MRLFGYARVSTSQQSLDIQV
RALKDAGVKANRIFTDKASG
SSSDRKGLDLLRMKVEEGDV
ILVKKLDRLGRDTADMIQLI
KEFDAQGVSIRFIDDGISTD
GEMGK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 696 |
| 13C chemical shifts | 411 |
| 15N chemical shifts | 109 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | resolvase | 1 |
Entities:
Entity 1, resolvase 105 residues - 11608 Da.
| 1 | MET | ARG | LEU | PHE | GLY | TYR | ALA | ARG | VAL | SER | ||||
| 2 | THR | SER | GLN | GLN | SER | LEU | ASP | ILE | GLN | VAL | ||||
| 3 | ARG | ALA | LEU | LYS | ASP | ALA | GLY | VAL | LYS | ALA | ||||
| 4 | ASN | ARG | ILE | PHE | THR | ASP | LYS | ALA | SER | GLY | ||||
| 5 | SER | SER | SER | ASP | ARG | LYS | GLY | LEU | ASP | LEU | ||||
| 6 | LEU | ARG | MET | LYS | VAL | GLU | GLU | GLY | ASP | VAL | ||||
| 7 | ILE | LEU | VAL | LYS | LYS | LEU | ASP | ARG | LEU | GLY | ||||
| 8 | ARG | ASP | THR | ALA | ASP | MET | ILE | GLN | LEU | ILE | ||||
| 9 | LYS | GLU | PHE | ASP | ALA | GLN | GLY | VAL | SER | ILE | ||||
| 10 | ARG | PHE | ILE | ASP | ASP | GLY | ILE | SER | THR | ASP | ||||
| 11 | GLY | GLU | MET | GLY | LYS |
Samples:
sample_one: resolvase, [U-15N], 2.0 mM; D2O 10%; H2O 90%
sample_two: resolvase, [U-13C; U-15N], 2.0 mM
sample_conditions_one: pH: 6.5; temperature: 298 K; ionic strength: 1.0
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | sample_conditions_one |
| HNCOCA | not available | not available | sample_conditions_one |
| HNCACB | not available | not available | sample_conditions_one |
| CBCACONH | not available | not available | sample_conditions_one |
| HNCO, | not available | not available | sample_conditions_one |
| CCONH | not available | not available | sample_conditions_one |
| HCCONH | not available | not available | sample_conditions_one |
| HCCH-TOCSY | not available | not available | sample_conditions_one |
| 1H-NOESY | not available | not available | sample_conditions_one |
Software:
FELIX v95 - Data Processing
XEASY v1.3.13 - Spectral visualization and resonance assignment
NMR spectrometers:
- Varian . 500 MHz
- Varian . 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA03915 BAA97879 |
| EMBL | CAA42759 CTC93933 CUH58641 |
| GB | AAZ05410 ABX47149 ABX47153 ACB02468 ACT33552 |
| PRF | 0812253A |
| REF | NP_061388 WP_001235704 WP_001749204 WP_005766999 WP_010129789 |
| SP | P03012 |
Download simulated HSQC data in one of the following formats:
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