BMRB Entry 4204
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4204
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Title: Three-Dimensional Structure of Human Insulin-Like Growth Factor-I (IGF-I) Determined by 1H-NMR and Distance Geometry
Deposition date: 1997-09-21 Original release date: 1999-12-22
Authors: Sato, A.; Nishimura, S.; Ohkubo, T.; Kyogoku, Y.; Koyama, S.; Kobayashi, M.; Kobayashi, T.
Citation: Sato, A.; Nishumura, S.; Ohkubo, T.; Kyogoku, Y.; Koyama, S.; Kobatashi, M.; Yasuda, T.; Kobayashi, Y.. "Three-Dimensional Structure of Human Insulin-like Growth Factor-I (IGF-I) Determined by 1H-NMR and Distance Geometry" Int. J. Pept. Protein Res. 41, 433-440 (1993).
Assembly members:
Insulin-like growth factor-I (Somatomedin C), polymer, 70 residues, 7649 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Insulin-like growth factor-I (Somatomedin C): GPETLCGAELVDALQFVCGD
RGFYFNKPTGYGSSSRRAPQ
TGIVDECCFRSCDLRRLEMY
CAPLKPAKSA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 459 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IGF-I | 1 |
Entities:
Entity 1, IGF-I 70 residues - 7649 Da.
| 1 | GLY | PRO | GLU | THR | LEU | CYS | GLY | ALA | GLU | LEU | |
| 2 | VAL | ASP | ALA | LEU | GLN | PHE | VAL | CYS | GLY | ASP | |
| 3 | ARG | GLY | PHE | TYR | PHE | ASN | LYS | PRO | THR | GLY | |
| 4 | TYR | GLY | SER | SER | SER | ARG | ARG | ALA | PRO | GLN | |
| 5 | THR | GLY | ILE | VAL | ASP | GLU | CYS | CYS | PHE | ARG | |
| 6 | SER | CYS | ASP | LEU | ARG | ARG | LEU | GLU | MET | TYR | |
| 7 | CYS | ALA | PRO | LEU | LYS | PRO | ALA | LYS | SER | ALA |
Samples:
sample_one: Insulin-like growth factor-I (Somatomedin C) 10 mM; D20 90%; acetic acid, [U-2H], 10%
sample_two: Insulin-like growth factor-I (Somatomedin C) 10 mM; D20 10%; H20 80%; acetic acid, [U-2H], 10%
sample_conditions_one: pH: 3.0; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| ph dependence experiment | sample_one | not available | sample_conditions_one |
| temperature dependence experiment | sample_one | not available | sample_conditions_one |
| H/D exchange experiment | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- JEOL GSX 500 MHz
Related Database Links:
| BMRB | 15654 2498 4069 4278 4494 |
| PDB | |
| DBJ | BAA01200 BAA01976 BAA07897 BAB77524 BAD92421 |
| EMBL | CAA24998 CAA27153 CAA28759 CAA33746 CAA35098 |
| GB | AAA31043 AAA31544 AAA31545 AAA31546 AAA31547 |
| PIR | S22878 |
| PRF | 0912651A 1001199A 1203258A 1707201A 2001274A |
| REF | NP_000609 NP_001009774 NP_001071296 NP_001075495 NP_001075967 |
| SP | P05019 P07455 P10763 P16545 P17647 |
| TPG | DAA29731 |