BMRB Entry 27146
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27146
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Title: Est3 Hansenula polymorpha telomerase subunit PubMed: 28916982
Deposition date: 2017-06-17 Original release date: 2017-09-29
Authors: Polshakov, Vladimir; Mariasina, Sofia; Efimov, Sergey; Petrova, Olga
Citation: Mariasina, Sofia; Efimov, Sergey; Petrova, Olga; Rodina, Elena; Malyavko, Alexander; Zvereva, Maria; Klochkov, Vladimir; Dontsova, Olga; Polshakov, Vladimir. "Chemical shift assignments and the secondary structure of the Est3 telomerase subunit in the yeast Hansenula polymorpha" Biomol. NMR Assignments 12, 57-62 (2018).
Assembly members:
Est3, polymer, 178 residues, Formula weight is not available
Natural source: Common Name: yeast Taxonomy ID: 870730 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Hansenula polymorpha
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Est3: GAMGPPSSRDAVRVTASAHM
KHWLEPVLCEAGLGHNYKVD
KVLKVLRIYPRSNTLSSLPL
CLCDANYKILAFANYKAIAA
FERKERRRVTQNLLNSEIMI
HSFTIRFYNDDQVQGFFDGL
KFKQKASLFPGYLVLEINDF
SMFNRDQLILSNAGTIEFLY
GTPRYIARFIEQEFSDEE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 676 |
| 15N chemical shifts | 183 |
| 1H chemical shifts | 1138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hpEst3 | 1 |
Entities:
Entity 1, hpEst3 178 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | PRO | PRO | SER | SER | ARG | ASP | ||||
| 2 | ALA | VAL | ARG | VAL | THR | ALA | SER | ALA | HIS | MET | ||||
| 3 | LYS | HIS | TRP | LEU | GLU | PRO | VAL | LEU | CYS | GLU | ||||
| 4 | ALA | GLY | LEU | GLY | HIS | ASN | TYR | LYS | VAL | ASP | ||||
| 5 | LYS | VAL | LEU | LYS | VAL | LEU | ARG | ILE | TYR | PRO | ||||
| 6 | ARG | SER | ASN | THR | LEU | SER | SER | LEU | PRO | LEU | ||||
| 7 | CYS | LEU | CYS | ASP | ALA | ASN | TYR | LYS | ILE | LEU | ||||
| 8 | ALA | PHE | ALA | ASN | TYR | LYS | ALA | ILE | ALA | ALA | ||||
| 9 | PHE | GLU | ARG | LYS | GLU | ARG | ARG | ARG | VAL | THR | ||||
| 10 | GLN | ASN | LEU | LEU | ASN | SER | GLU | ILE | MET | ILE | ||||
| 11 | HIS | SER | PHE | THR | ILE | ARG | PHE | TYR | ASN | ASP | ||||
| 12 | ASP | GLN | VAL | GLN | GLY | PHE | PHE | ASP | GLY | LEU | ||||
| 13 | LYS | PHE | LYS | GLN | LYS | ALA | SER | LEU | PHE | PRO | ||||
| 14 | GLY | TYR | LEU | VAL | LEU | GLU | ILE | ASN | ASP | PHE | ||||
| 15 | SER | MET | PHE | ASN | ARG | ASP | GLN | LEU | ILE | LEU | ||||
| 16 | SER | ASN | ALA | GLY | THR | ILE | GLU | PHE | LEU | TYR | ||||
| 17 | GLY | THR | PRO | ARG | TYR | ILE | ALA | ARG | PHE | ILE | ||||
| 18 | GLU | GLN | GLU | PHE | SER | ASP | GLU | GLU |
Samples:
sample_CN: Est3, [U-99% 13C; U-99% 15N], 0.5 mM; potassium chloride 100 mM; sodium phosphate 50 mM; DTT 3 mM; sodium azide 0.02%
sample_CN_D2O: Est3, [U-99% 13C; U-99% 15N], 0.7 mM; potassium chloride 100 mM; sodium phosphate 50 mM; DTT 3 mM; sodium azide 0.02%
Sample_N: Est3, [U-99% 15N], 0.8 mM; potassium chloride 100 mM; sodium phosphate 50 mM; DTT 3 mM; sodium azide 0.02%
sample_unl: Est3 1.0 mM; potassium chloride 100 mM; sodium phosphate 50 mM; DTT 3 mM; sodium azide 0.02%
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | Sample_N | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_CN_D2O | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_CN_D2O | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_unl | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_unl | isotropic | sample_conditions_2 |
| 3D HNCO | sample_CN | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_CN | isotropic | sample_conditions_1 |
| 3D HNCA | sample_CN | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_CN | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_CN | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_CN | isotropic | sample_conditions_1 |
| 3D HNHA | Sample_N | isotropic | sample_conditions_1 |
| 3D HNHB | Sample_N | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_CN_D2O | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_CN_D2O | isotropic | sample_conditions_1 |
| 2D HBCB(CGCD)HD | sample_CN_D2O | isotropic | sample_conditions_1 |
| 2D HBCB(CGCDCE)HE | sample_CN_D2O | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | Sample_N | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | Sample_N | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | Sample_N | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.1, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis, peak picking
PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment
AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMRest, Polshakov - chemical shift calculation, data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts