BMRB Entry 25849
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25849
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Title: NMR Structure of the homeodomain transcription factor Gbx1[E17R,R52E} from Homo sapiens PubMed: 27396829
Deposition date: 2015-10-15 Original release date: 2015-10-26
Authors: Proudfoot, Andrew; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt
Citation: Proudfoot, Andrew; Geralt, Michael; Elsliger, Marc-Andre; Wilson, Ian; Wuthrich, Kurt; Serrano, Pedro. "Dynamic Local Polymorphisms in the Gbx1 Homeodomain Induced by DNA Binding" Structure 24, 1372-1379 (2016).
Assembly members:
entity, polymer, 71 residues, 8316.757 Da.
Natural source: Common Name: humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GAPGGKSRRRRTAFTSEQLL
ELRKEFHCKKYLSLTERSQI
AHALKLSEVQVKIWFQNERA
KWKRIKAGNVS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 298 |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 462 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 71 residues - 8316.757 Da.
| 1 | GLY | ALA | PRO | GLY | GLY | LYS | SER | ARG | ARG | ARG | ||||
| 2 | ARG | THR | ALA | PHE | THR | SER | GLU | GLN | LEU | LEU | ||||
| 3 | GLU | LEU | ARG | LYS | GLU | PHE | HIS | CYS | LYS | LYS | ||||
| 4 | TYR | LEU | SER | LEU | THR | GLU | ARG | SER | GLN | ILE | ||||
| 5 | ALA | HIS | ALA | LEU | LYS | LEU | SER | GLU | VAL | GLN | ||||
| 6 | VAL | LYS | ILE | TRP | PHE | GLN | ASN | GLU | ARG | ALA | ||||
| 7 | LYS | TRP | LYS | ARG | ILE | LYS | ALA | GLY | ASN | VAL | ||||
| 8 | SER |
Samples:
sample_1: entity, [U-99% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.0798 M; pH: 6.0; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
DYANA, G??ntert P. - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts