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BMRB Entry 25802

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25802
MolProbity Validation Chart

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Title: Resonance assignments of [15N, 13C] human DCL-1 (CD302) extracellular domain   PubMed: 26725057

Deposition date: 2015-09-09 Original release date: 2016-01-06

Authors: Pospisilova, Eliska; Kavan, Daniel; Novak, Petr; Chmelik, Josef

Citation: Pospisilova, Eliska; Kavan, Daniel; Novak, Petr; Chmelik, Josef. "1H, 13C and 15N resonance assignments of human DCL-1 (CD302) extracellular domain"  Biomol. NMR Assign. 10, 189-192 (2016).

Assembly members:
CD302, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CD302: MDCPSSTWIQFQDSCYIFLQ EAIKVESIEDVRNQCTDHGA DMISIHNEEENAFILDTLKK QWKGPDDILLGMFYDTDDAS FKWFDNSNMTFDKWTDQDDD EDLVDTCAFLHIKTGEWKKG NCEVSSVEGTLCKTAIPYKR

Data sets:
Data typeCount
13C chemical shifts627
15N chemical shifts151
1H chemical shifts962

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CD3021

Entities:

Entity 1, CD302 140 residues - Formula weight is not available

Construct contains initial MET (MET22) which is not in full native sequence.

1   METASPCYSPROSERSERTHRTRPILEGLN
2   PHEGLNASPSERCYSTYRILEPHELEUGLN
3   GLUALAILELYSVALGLUSERILEGLUASP
4   VALARGASNGLNCYSTHRASPHISGLYALA
5   ASPMETILESERILEHISASNGLUGLUGLU
6   ASNALAPHEILELEUASPTHRLEULYSLYS
7   GLNTRPLYSGLYPROASPASPILELEULEU
8   GLYMETPHETYRASPTHRASPASPALASER
9   PHELYSTRPPHEASPASNSERASNMETTHR
10   PHEASPLYSTRPTHRASPGLNASPASPASP
11   GLUASPLEUVALASPTHRCYSALAPHELEU
12   HISILELYSTHRGLYGLUTRPLYSLYSGLY
13   ASNCYSGLUVALSERSERVALGLUGLYTHR
14   LEUCYSLYSTHRALAILEPROTYRLYSARG

Samples:

sample_1: CD302, [U-100% 13C; U-100% 15N], 0.5 mM; PIPES 25 mM; sodium chloride 50 mM; sodium azide 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D H(CC)(CO)NHsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 600 MHz

Related Database Links:

UNP Q8IX05

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts