BMRB Entry 19484
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19484
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Title: NMR structure of a two-transmembrane segment TM VI-VII of NHE1 PubMed: 24840010
Deposition date: 2013-09-10 Original release date: 2014-06-30
Authors: Lee, Brian; Sykes, Brian; Alves, Claudia; Fliegel, Larry
Citation: Alves, Claudia; Lee, Brian; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of the Transmembrane Segment Pair VI and VII of the NHE1 Isoform of the Na(+)/H(+) Exchanger" Biochemistry 53, 3658-3670 (2014).
Assembly members:
entity, polymer, 57 residues, 6257.431 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GSKKKDNLLFGSIISAVDPV
AVLAVFEEIHINELLHILVF
GESLLNDAVTVVLYKKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 393 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tm67 | 1 |
Entities:
Entity 1, tm67 57 residues - 6257.431 Da.
| 1 | GLY | SER | LYS | LYS | LYS | ASP | ASN | LEU | LEU | PHE | ||||
| 2 | GLY | SER | ILE | ILE | SER | ALA | VAL | ASP | PRO | VAL | ||||
| 3 | ALA | VAL | LEU | ALA | VAL | PHE | GLU | GLU | ILE | HIS | ||||
| 4 | ILE | ASN | GLU | LEU | LEU | HIS | ILE | LEU | VAL | PHE | ||||
| 5 | GLY | GLU | SER | LEU | LEU | ASN | ASP | ALA | VAL | THR | ||||
| 6 | VAL | VAL | LEU | TYR | LYS | LYS | LYS |
Samples:
sample_1: entity, [U-15N], 1 – 2 mM; D2O, [U-2H], 5%; DSS, [1,1,2,2,3,3-2H6], 0.25 mM; H2O 95%; dodecylphosphocholine, [U-2H], 75 – 150 mM
sample_2: entity, [U-15N], 1 – 2 mM; D2O, [U-2H], 100%; DSS, [1,1,2,2,3,3-2H6], 0.25 mM; dodecylphosphocholine, [U-2H], 75 – 150 mM
sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE02258 BAE29332 BAE32882 BAE38221 BAE75800 |
| EMBL | CAA42558 CAA43721 CAA69925 CAB45232 CAF94405 |
| GB | AAA31092 AAA91483 AAA92976 AAA98479 AAB20633 |
| REF | NP_001007104 NP_001038108 NP_001081553 NP_001095191 NP_001273957 |
| SP | P19634 P23791 P26431 P48762 Q28036 |
| TPG | DAA32066 |