BMRB Entry 19416
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19416
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Title: NMR Structure of Rrp7 C-terminal Domain PubMed: 24130456
Deposition date: 2013-08-08 Original release date: 2013-09-23
Authors: Lin, Jinzhong; Feng, Yingang; Ye, Keqiong
Citation: Lin, Jinzhong; Lu, Jing; Feng, Yingang; Sun, Mengyi; Ye, Keqiong. "An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme." PLoS Biol. 11, .-. (2013).
Assembly members:
entity, polymer, 46 residues, 5732.697 Da.
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GPEADFYRFQVRERKKQEIN
QLLSKFKEDQERIKVMKAKR
KFNPYT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 218 |
| 15N chemical shifts | 49 |
| 1H chemical shifts | 350 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rrp7 C-terminal Domain | 1 |
Entities:
Entity 1, Rrp7 C-terminal Domain 46 residues - 5732.697 Da.
| 1 | GLY | PRO | GLU | ALA | ASP | PHE | TYR | ARG | PHE | GLN | ||||
| 2 | VAL | ARG | GLU | ARG | LYS | LYS | GLN | GLU | ILE | ASN | ||||
| 3 | GLN | LEU | LEU | SER | LYS | PHE | LYS | GLU | ASP | GLN | ||||
| 4 | GLU | ARG | ILE | LYS | VAL | MET | LYS | ALA | LYS | ARG | ||||
| 5 | LYS | PHE | ASN | PRO | TYR | THR |
Samples:
sample_1: entity, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; sodium azide 0.05%
sample_conditions_1: ionic strength: 0.05 M; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
FELIX, Accelrys Software Inc. - processing
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| GB | NP_009899 AHN96030 AHV79235 AHY79656 AJP37407 AJQ31893 |
| PDB | |
| DBJ | GAA21887 |
| EMBL | CAA42385 CAY78174 |
| REF | NP_009899 |
| SP | P25368 |
| TPG | DAA07453 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts