BMRB Entry 17953
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17953
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Title: NMR Structure of protoporphyrin-IX bound murine p22HBP
Deposition date: 2011-09-22 Original release date: 2012-09-24
Authors: Goodfellow, Brian; Dias, J.; Macedo, A.; Ferreira, G.; Peterson, F.; Volkman, B.; Duarte, I.
Citation: Goodfellow, Brian; Dias, J.; Macedo, A.; Ferreira, G.; Peterson, F.; Volkman, B.; Duarte, I.. "The NMR Structure of protoporphyrin-IX bound murine p22HBP" to be published ., .-..
Assembly members:
HEME-BINDING_PROTEIN_1, polymer, 195 residues, 21737.3322 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HEME-BINDING_PROTEIN_1: MKNSTHHHHHHNSLFGSVET
WPWQVLSTGGKEDVSYEERA
CEGGKFATVEVTDKPVDEAL
REAMPKIMKYVGGTNDKGVG
MGMTVPVSFAVFPNEDGSLQ
KKLKVWFRIPNQFQGSPPAP
SDESVKIEEREGITVYSTQF
GGYAKEADYVAHATQLRTTL
EGTPATYQGDVYYCAGYDPP
MKPYGRRNEVWLVKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 639 |
| 15N chemical shifts | 166 |
| 1H chemical shifts | 1031 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HEME-BINDING PROTEIN 1 | 1 |
Entities:
Entity 1, HEME-BINDING PROTEIN 1 195 residues - 21737.3322 Da.
| 1 | MET | LYS | ASN | SER | THR | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | ASN | SER | LEU | PHE | GLY | SER | VAL | GLU | THR | ||||
| 3 | TRP | PRO | TRP | GLN | VAL | LEU | SER | THR | GLY | GLY | ||||
| 4 | LYS | GLU | ASP | VAL | SER | TYR | GLU | GLU | ARG | ALA | ||||
| 5 | CYS | GLU | GLY | GLY | LYS | PHE | ALA | THR | VAL | GLU | ||||
| 6 | VAL | THR | ASP | LYS | PRO | VAL | ASP | GLU | ALA | LEU | ||||
| 7 | ARG | GLU | ALA | MET | PRO | LYS | ILE | MET | LYS | TYR | ||||
| 8 | VAL | GLY | GLY | THR | ASN | ASP | LYS | GLY | VAL | GLY | ||||
| 9 | MET | GLY | MET | THR | VAL | PRO | VAL | SER | PHE | ALA | ||||
| 10 | VAL | PHE | PRO | ASN | GLU | ASP | GLY | SER | LEU | GLN | ||||
| 11 | LYS | LYS | LEU | LYS | VAL | TRP | PHE | ARG | ILE | PRO | ||||
| 12 | ASN | GLN | PHE | GLN | GLY | SER | PRO | PRO | ALA | PRO | ||||
| 13 | SER | ASP | GLU | SER | VAL | LYS | ILE | GLU | GLU | ARG | ||||
| 14 | GLU | GLY | ILE | THR | VAL | TYR | SER | THR | GLN | PHE | ||||
| 15 | GLY | GLY | TYR | ALA | LYS | GLU | ALA | ASP | TYR | VAL | ||||
| 16 | ALA | HIS | ALA | THR | GLN | LEU | ARG | THR | THR | LEU | ||||
| 17 | GLU | GLY | THR | PRO | ALA | THR | TYR | GLN | GLY | ASP | ||||
| 18 | VAL | TYR | TYR | CYS | ALA | GLY | TYR | ASP | PRO | PRO | ||||
| 19 | MET | LYS | PRO | TYR | GLY | ARG | ARG | ASN | GLU | VAL | ||||
| 20 | TRP | LEU | VAL | LYS | ALA |
Samples:
sample_1: HEME-BINDING_PROTEIN_1, [U-13C; U-15N], 1 mM
sample_conditions_1: ionic strength: 150.000 mM; pH: 8.000; pressure: 1.000 atm; temperature: 303.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D/3D 15N NOESY | sample_1 | solution | sample_conditions_1 |
| 2D/3D 13C NOESY (aliphatic and aromatic) | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, AutoDep -
CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| UNP | HEBP1_MOUSE |
| PDB | |
| DBJ | BAA33770 |
| EMBL | CAJ18470 |
| GB | AAD32096 AAH12654 AAI68221 EDL10544 EDM01631 |
| REF | NP_038574 XP_006237591 XP_011239526 |
| SP | Q9R257 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts