BMRB Entry 17682

Name: The 912-888 alternate conformation for helix 27 of E.coli 16S rRNA
Published: 2011-09-01

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PDB ID: 2ldt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17682
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: The 912-888 alternate conformation for helix 27 of E.coli 16S rRNA PubMed: 21442607

Deposition date: 2011-06-02 Original release date: 2011-09-01

Authors: Spano, Meredith; Walter, Nils

Citation: Spano, Meredith Newby; Walter, Nils. "Solution structure of an alternate conformation of helix27 from Escherichia coli16S rRNA." Biopolymers 95, 653-668 (2011).

Assembly members:
RNA_(31-MER), polymer, 31 residues, 3775.495 Da.

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: enzymatic semisynthesis Host organism: Escherichia coli

Entity Sequences (FASTA):
RNA_(31-MER): GGGGAGUACGGCCGCAAGGU UAAAACUCCCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	39
15N chemical shifts	8
1H chemical shifts	157

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA (31-MER)	1

Entities:

Entity 1, RNA (31-MER) 31 residues - 3775.495 Da.

1

G

A

G

U

A

C

G

2

G

C

G

C

A

G

U

3

U

A

C

U

C

4

C

Samples:

sample_2: RNA (31-MER), [U-99% 13C; U-99% 15N], 0.8 – 1.6 mM; H2O 90%; D2O 10%; NaPi 10 mM; EDTA 0.1 mM; NaCl 50 mM

sample_conditions_1: ionic strength: .05 M; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 800 MHz
Bruker Avance 600 MHz