BMRB Entry 16191

Name: NMR-Assignments of a Cytosolic Domain of the C-Terminus of Polycystin-2
Published: 2009-04-16

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PDB ID: 2kle
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16191
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR-Assignments of a Cytosolic Domain of the C-Terminus of Polycystin-2 PubMed: 19636966

Deposition date: 2009-03-02 Original release date: 2009-04-16

Authors: Kalbitzer, Hans Robert

Citation: Schumann, Frank; Hoffmeister, Helen; Schmidt, Maren; Bader, Reto; Besl, Elisabeth; Witzgall, Ralph; Kalbitzer, Hans Robert. "NMR-assignments of a cytosolic domain of the C-terminus of polycystin-2" Biomol. NMR Assignments 3, 141-144 (2009).

Assembly members:
Polycystin-2_Polypeptide, polymer, 123 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Polycystin-2_Polypeptide: GSTAIGINDTYSEVKSDLAQ QKAEMELSDLIRKGYHKALV KLKLKKNTVDDISESLRQGG GKLNFDELRQDLKGKGHTDA EIEAIFTKYDQDGDQELTEH EHQQMRDDLEKEREDLDLDH SSL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	348
15N chemical shifts	100
1H chemical shifts	569

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Polycystin-2 Polypeptide	1
2	Calcium	2

Entities:

Entity 1, Polycystin-2 Polypeptide 123 residues - Formula weight is not available

1

GLY

SER

THR

ALA

ILE

GLY

ILE

ASN

ASP

THR

2

TYR

SER

GLU

VAL

LYS

SER

ASP

LEU

ALA

GLN

3

GLN

LYS

ALA

GLU

MET

GLU

LEU

SER

ASP

LEU

4

ILE

ARG

LYS

GLY

TYR

HIS

LYS

ALA

LEU

VAL

5

LYS

LEU

LYS

LEU

LYS

ASN

THR

VAL

ASP

6

ASP

ILE

SER

GLU

SER

LEU

ARG

GLN

GLY

7

GLY

LYS

LEU

ASN

PHE

ASP

GLU

LEU

ARG

GLN

8

ASP

LEU

LYS

GLY

LYS

GLY

HIS

THR

ASP

ALA

9

GLU

ILE

GLU

ALA

ILE

PHE

THR

LYS

TYR

ASP

10

GLN

ASP

GLY

ASP

GLN

GLU

LEU

THR

GLU

HIS

11

GLU

HIS

GLN

MET

ARG

ASP

LEU

GLU

12

LYS

GLU

ARG

GLU

ASP

LEU

ASP

LEU

ASP

HIS

13

SER

LEU

Entity 2, Calcium - Ca - 40.078 Da.

1

CA

Samples:

sample_1: Polycystin-2 Polypeptide, [U-100% 13C; U-100% 15N], 0.5 mM; Ca2 5 mM; DSS 0.1 mM; potassium phosphate buffer 10 mM; NaCl 500 mM; DTE 2 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 0.51 M; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCANNH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 15N-TOCSY-HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N-NOESY-HSQC	sample_1	isotropic	sample_conditions_1

Software:

AUREMOL, Bruker Biospin - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

BMRB	16590 17621 18268
PDB	2KLD 2KLE 2KQ6 2Y4Q
DBJ	BAG56956 BAG57494
EMBL	CAI38797
GB	AAC16004 AAC50520 AAC50933 AAI11455 AAI12262
REF	NP_000288 NP_001039777 NP_001232908 XP_001099242 XP_002717056
SP	Q13563 Q4GZT3
TPG	DAA28806

Biological Magnetic Resonance Data Bank