BMRB Entry 15312
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15312
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Title: Solution structure of NusB from Aquifex Aeolicus PubMed: 18177898
Deposition date: 2007-06-18 Original release date: 2008-02-28
Authors: Das, Ranabir; Loss, Sandra; Li, Jess; Tarasov, Sergey; Wingfield, Paul; Waugh, David; Byrd, Robert; Altieri, Amanda
Citation: Das, Ranabir; Loss, Sandra; Li, Jess; Waugh, David; Tarasov, Sergey; Wingfield, Paul; Byrd, Robert; Altieri, Amanda. "Structural biophysics of the NusB:NusE antitermination complex" J. Mol. Biol. 376, 705-720 (2008).
Assembly members:
NusB, polymer, 148 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 63363 Superkingdom: Bacteria Kingdom: not available Genus/species: Aquifex aeolicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NusB: MRYRKGARDTAFLVLYRWDL
RGENPGELFKEVVEEKNIKN
KDAYEYAKKLVDTAVRHIEE
IDSIIEKHLKGWSIDRLGYV
ERNALRLGVAELIFLKSKEP
GRVFIDIVDLVKKYADEKAG
KFVNGVLSAIYKAYITSSKE
EKPSLKSE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 656 |
| 15N chemical shifts | 143 |
| 1H chemical shifts | 1030 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Aquifex NusB monomer | 1 |
Entities:
Entity 1, Aquifex NusB monomer 148 residues - Formula weight is not available
| 1 | MET | ARG | TYR | ARG | LYS | GLY | ALA | ARG | ASP | THR | ||||
| 2 | ALA | PHE | LEU | VAL | LEU | TYR | ARG | TRP | ASP | LEU | ||||
| 3 | ARG | GLY | GLU | ASN | PRO | GLY | GLU | LEU | PHE | LYS | ||||
| 4 | GLU | VAL | VAL | GLU | GLU | LYS | ASN | ILE | LYS | ASN | ||||
| 5 | LYS | ASP | ALA | TYR | GLU | TYR | ALA | LYS | LYS | LEU | ||||
| 6 | VAL | ASP | THR | ALA | VAL | ARG | HIS | ILE | GLU | GLU | ||||
| 7 | ILE | ASP | SER | ILE | ILE | GLU | LYS | HIS | LEU | LYS | ||||
| 8 | GLY | TRP | SER | ILE | ASP | ARG | LEU | GLY | TYR | VAL | ||||
| 9 | GLU | ARG | ASN | ALA | LEU | ARG | LEU | GLY | VAL | ALA | ||||
| 10 | GLU | LEU | ILE | PHE | LEU | LYS | SER | LYS | GLU | PRO | ||||
| 11 | GLY | ARG | VAL | PHE | ILE | ASP | ILE | VAL | ASP | LEU | ||||
| 12 | VAL | LYS | LYS | TYR | ALA | ASP | GLU | LYS | ALA | GLY | ||||
| 13 | LYS | PHE | VAL | ASN | GLY | VAL | LEU | SER | ALA | ILE | ||||
| 14 | TYR | LYS | ALA | TYR | ILE | THR | SER | SER | LYS | GLU | ||||
| 15 | GLU | LYS | PRO | SER | LEU | LYS | SER | GLU |
Samples:
sample_1: Aquifex NusB monomer, [U-13C; U-15N], 0.7 mM; potassium phosphate 50 mM; potassium chloride 200 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 308.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 4D 15N/13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 4D 13C/13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
ANSIG, Kraulis - chemical shift assignment
SPARKY, Goddard - peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts