BMRB Entry 15110
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15110
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Title: Solution structure of V21C/V59C Lymphotactin/XCL1 PubMed: 17302442
Deposition date: 2007-01-23 Original release date: 2007-05-04
Authors: Volkman, B.; Tuinstra, R.; Peterson, F.; Elgin, E.
Citation: Tuinstra, R.; Peterson, F.; Elgin, E.; Pelzek, A.; Volkman, B.. "An engineered second disulfide bond restricts lymphotactin/XCL1 to a chemokine-like conformation with XCR1 agonist activity" Biochemistry 46, 2564-2573 (2007).
Assembly members:
Lymphotactin, polymer, 92 residues, 10198.776 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Lymphotactin: GSEVSDKRTCVSLTTQRLPC
SRIKTYTITEGSLRAVIFIT
KRGLKVCADPQATWVRDCVR
SMDRKSNTRNNMIQTKPTGT
QQSTNTAVTLTG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 377 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 593 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lymphotactin | 1 |
Entities:
Entity 1, Lymphotactin 92 residues - 10198.776 Da.
| 1 | GLY | SER | GLU | VAL | SER | ASP | LYS | ARG | THR | CYS | ||||
| 2 | VAL | SER | LEU | THR | THR | GLN | ARG | LEU | PRO | CYS | ||||
| 3 | SER | ARG | ILE | LYS | THR | TYR | THR | ILE | THR | GLU | ||||
| 4 | GLY | SER | LEU | ARG | ALA | VAL | ILE | PHE | ILE | THR | ||||
| 5 | LYS | ARG | GLY | LEU | LYS | VAL | CYS | ALA | ASP | PRO | ||||
| 6 | GLN | ALA | THR | TRP | VAL | ARG | ASP | CYS | VAL | ARG | ||||
| 7 | SER | MET | ASP | ARG | LYS | SER | ASN | THR | ARG | ASN | ||||
| 8 | ASN | MET | ILE | GLN | THR | LYS | PRO | THR | GLY | THR | ||||
| 9 | GLN | GLN | SER | THR | ASN | THR | ALA | VAL | THR | LEU | ||||
| 10 | THR | GLY |
Samples:
sample: Lymphotactin, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_15N-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY (AROMATIC) | sample | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker Biospin - collection
NMRPipe v2004, Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A. - processing
XEASY v1.3, Bartels, C, Xia, T.-H., Billeter, M., Guntert, P., Wuthrich, K. - data analysis
GARANT v2.1, Bartels, C., Billeter, M., Guntert, P., Wuthrich, K. - data analysis
CYANA v2.1, Herrmann, T., Guntert, P., Wuthrich, K. - structure solution
X-PLOR NIH v2.9.3, Schwieters, C.D., Kuszewski, J.J, Tjandra, N., Clore, G.M. - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 15215 |
| PDB | |
| DBJ | BAA07825 BAA09859 |
| EMBL | CAA60198 |
| GB | AAC50164 AAH69817 AAH70309 AIC49701 AJA36131 |
| REF | NP_002986 XP_003824662 XP_004027907 XP_009436138 XP_011508167 |
| SP | P47992 |
Download simulated HSQC data in one of the following formats:
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