BMRB Entry 11321
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11321
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Title: Solution Structure of the IBR domain of the RING finger protein 31 protein
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Koshiba, S.; Tomizawa, T.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Koshiba, S.; Tomizawa, T.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the IBR domain of the RING finger protein 31 protein" . ., .-..
Assembly members:
IBR domain, polymer, 86 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
IBR domain: GSSGSSGALFHKKLTEGVLM
RDPKFLWCAQCSFGFIYERE
QLEATCPQCHQTFCVRCKRQ
WEEQHRGRSCEDFQNWKRMN
SGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 355 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 540 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IBR domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, IBR domain 86 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | LEU | PHE | ||||
| 2 | HIS | LYS | LYS | LEU | THR | GLU | GLY | VAL | LEU | MET | ||||
| 3 | ARG | ASP | PRO | LYS | PHE | LEU | TRP | CYS | ALA | GLN | ||||
| 4 | CYS | SER | PHE | GLY | PHE | ILE | TYR | GLU | ARG | GLU | ||||
| 5 | GLN | LEU | GLU | ALA | THR | CYS | PRO | GLN | CYS | HIS | ||||
| 6 | GLN | THR | PHE | CYS | VAL | ARG | CYS | LYS | ARG | GLN | ||||
| 7 | TRP | GLU | GLU | GLN | HIS | ARG | GLY | ARG | SER | CYS | ||||
| 8 | GLU | ASP | PHE | GLN | ASN | TRP | LYS | ARG | MET | ASN | ||||
| 9 | SER | GLY | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: IBR domain, [U-13C; U-15N], 1.55 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.925, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB15675 BAB70948 BAB84970 BAF35583 BAF83936 |
| GB | AAH09821 AAH12077 AAH17376 AAP12522 AIC60278 |
| REF | NP_001297261 NP_060469 XP_001112195 XP_001166671 XP_001490713 |
| SP | Q96EP0 |
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