BMRB Entry 11054
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR11054
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Title: Solution structure of a novel insect chemokine isolated from integument PubMed: 19375321
Deposition date: 2008-07-27 Original release date: 2009-10-14
Authors: Kamiya, Masakatsu; Nakatogawa, Shin-ichi; Oda, Yasunori; Kamijima, Tatsuro; Aizawa, Tomoyasu; Demura, Makoto; Hayakawa, Yoichi; Kawano, Keiichi
Citation: Nakatogawa, Shin-ichi; Oda, Yasunori; Kamiya, Masakatsu; Kamijima, Tatsuro; Aizawa, Tomoyasu; Clark, Kevin; Demura, Makoto; Kawano, Keiichi; Strand, Michael; Hayakawa, Yoichi. "A novel peptide mediates aggregation and migration of hemocytes from an insect" Curr. Biol. 19, 779-785 (2009).
Assembly members:
insect chemokine, polymer, 32 residues, 3545.022 Da.
Natural source: Common Name: northern armyworm Taxonomy ID: 271217 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mythimna separata
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
insect chemokine: SVQILRCPDGMQMLRSGQCV
ATTEPPFDPDSY
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 32 |
| 1H chemical shifts | 158 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | insect chemokine | 1 |
Entities:
Entity 1, insect chemokine 32 residues - 3545.022 Da.
| 1 | SER | VAL | GLN | ILE | LEU | ARG | CYS | PRO | ASP | GLY | ||||
| 2 | MET | GLN | MET | LEU | ARG | SER | GLY | GLN | CYS | VAL | ||||
| 3 | ALA | THR | THR | GLU | PRO | PRO | PHE | ASP | PRO | ASP | ||||
| 4 | SER | TYR |
Samples:
sample_1: insect chemokine1 – 3 mM; DSS 10 uM
sample_2: entity, [U-15N], 1 – 3 mM; DSS 10 uM
sample_conditions_1: ionic strength: 0 M; pH: 5.1; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_2 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
Molmol, Koradi, Billeter and Wuthrich - data analysis
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
ProcheckNMR, Laskowski and MacArthur - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- Bruker DRX 500 MHz
- JEOL ECA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts