BMRB Entry 10213
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10213
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the GUCT domain from human ATP-dependent RNA helicase DDX50, DEAD box protein 50 PubMed: 18615715
Deposition date: 2008-04-09 Original release date: 2008-08-19
Authors: Ohnishi, S.; Paakkonen, K.; Guntert, P.; Sato, M.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.
Citation: Ohnishi, Satoshi; Paakkonen, Kimmo; Koshiba, Seizo; Tochio, Naoya; Sato, Manami; Kobayashi, Naohiro; Harada, Takushi; Watanabe, Satoru; Muto, Yutaka; Guntert, Peter; Tanaka, Akiko; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structure of the GUCT domain from human RNA helicase II/Gubeta reveals the RRM fold, but implausible RNA interactions." Proteins 74, 133-144 (2008).
Assembly members:
GUCT domain, polymer, 92 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: Cell free synthesis
Entity Sequences (FASTA):
GUCT domain: GSSGSSGFEPRSLITSDKGF
VTMTLESLEEIQDVSCAWKE
LNRKLSSNAVSQITRMCLLK
GNMGVCFDVPTTESERLQAE
WHDSDWILSVPA
- assigned_chemical_shifts
- RDCs
| Data type | Count |
| 13C chemical shifts | 387 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 603 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GUCT domain | 1 |
Entities:
Entity 1, GUCT domain 92 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PHE | GLU | PRO | ||||
| 2 | ARG | SER | LEU | ILE | THR | SER | ASP | LYS | GLY | PHE | ||||
| 3 | VAL | THR | MET | THR | LEU | GLU | SER | LEU | GLU | GLU | ||||
| 4 | ILE | GLN | ASP | VAL | SER | CYS | ALA | TRP | LYS | GLU | ||||
| 5 | LEU | ASN | ARG | LYS | LEU | SER | SER | ASN | ALA | VAL | ||||
| 6 | SER | GLN | ILE | THR | ARG | MET | CYS | LEU | LEU | LYS | ||||
| 7 | GLY | ASN | MET | GLY | VAL | CYS | PHE | ASP | VAL | PRO | ||||
| 8 | THR | THR | GLU | SER | GLU | ARG | LEU | GLN | ALA | GLU | ||||
| 9 | TRP | HIS | ASP | SER | ASP | TRP | ILE | LEU | SER | VAL | ||||
| 10 | PRO | ALA |
Samples:
sample_1: GUCT domain, [U-13C; U-15N], 0.9 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9816, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - structure solution
OPALp, Koradi, R. - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAG57047 BAG62959 |
| EMBL | CAB70733 |
| GB | AAH00210 AAH00272 AAH18637 AAK29402 ABM83473 |
| REF | NP_001245111 NP_076950 XP_002756344 XP_002820928 XP_003258245 |
| SP | Q9BQ39 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts