BMRB Entry 7365
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7365
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Title: Solution structure of Fe65 WW domain
Deposition date: 2007-01-19 Original release date: 2009-10-15
Authors: Kouno, T.; Mizuguchi, M.; Nozawa, Y.; Sugaya, T.; Kawano, K.
Citation: Kouno, T.; Mizuguchi, M.; Nozawa, Y.; Sugaya, T.; Kawano, K.. "Solution structure of Fe65 WW domain" to be published ., .-..
Assembly members:
Amyloid_beta_A4_precursor_protein-binding_family_B_member_1, polymer, 55 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Amyloid_beta_A4_precursor_protein-binding_family_B_member_1: GPLGSDSFWNPNAFETDSDL
PAGWMRVQDTSGTYYWHIPT
GTTQWEPPGRASPSQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 131 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 313 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WW domain | 1 |
Entities:
Entity 1, WW domain 55 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | ASP | SER | PHE | TRP | ASN | ||||
| 2 | PRO | ASN | ALA | PHE | GLU | THR | ASP | SER | ASP | LEU | ||||
| 3 | PRO | ALA | GLY | TRP | MET | ARG | VAL | GLN | ASP | THR | ||||
| 4 | SER | GLY | THR | TYR | TYR | TRP | HIS | ILE | PRO | THR | ||||
| 5 | GLY | THR | THR | GLN | TRP | GLU | PRO | PRO | GLY | ARG | ||||
| 6 | ALA | SER | PRO | SER | GLN |
Samples:
sample_1: WW domain, [U-13C; U-15N], 0.65 mM; phosphate buffer 10 mM; D2O 10%; H2O 90%
sample_2: WW domain, [U-13C; U-15N], 0.65 mM; phosphate buffer 10 mM; D2O 100%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_15N-separated_NOESY | sample_1 | isotropic | sample_conditions_1 |
| HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D_13C-separated_NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.2, F. Delaglio, et al. - processing
PIPP v4.3.2, D.S. Garret - collection
X-PLOR v3.1f, A.T. Brunger - structure solution
X-PLOR v3.1f, A.T. Brunger - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAH11531 BAH11532 BAH11554 BAH11576 BAH11788 |
| EMBL | CAA42998 CAD98057 |
| GB | AAK18816 ABL07489 EPY80480 KFO24725 |
| REF | NP_001240816 NP_001244248 NP_001244249 NP_001244250 NP_001244252 |
Download simulated HSQC data in one of the following formats:
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