BMRB Entry 7339
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7339
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Title: Solution structure of Arabidopsis thaliana protein At1g70830, a member of the major latex protein family PubMed: 19326460
Deposition date: 2006-11-09 Original release date: 2010-11-16
Authors: Volkman, B.; de la Cruz, N.; Lytle, B.; Peterson, F.
Citation: Lytle, Betsy; Song, Jikui; de la Cruz, Norberto; Peterson, Francis; Johnson, Kenneth; Bingman, Craig; Phillips, George; Volkman, Brian. "Structures of two Arabidopsis thaliana major latex proteins represent novel helix-grip folds." Proteins 76, 237-243 (2009).
Assembly members:
MLP-like_protein_28, polymer, 166 residues, Formula weight is not available
Natural source: Common Name: Mouse-ear cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
MLP-like_protein_28: GHHHHHHLETEASSLVGKLE
TDVEIKASADKFHHMFAGKP
HHVSKASPGNIQGCDLHEGD
WGTVGSIVFWNYVHDGEAKV
AKERIEAVEPDKNLITFRVI
EGDLMKEYKSFLLTIQVTPK
PGGPGSIVHWHLEYEKISEE
VAHPETLLQFCVEVSKEIDE
HLLAEE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 665 |
| 15N chemical shifts | 156 |
| 1H chemical shifts | 1047 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MLP-like protein 28 | 1 |
Entities:
Entity 1, MLP-like protein 28 166 residues - Formula weight is not available
| 1 | GLY | HIS | HIS | HIS | HIS | HIS | HIS | LEU | GLU | THR | ||||
| 2 | GLU | ALA | SER | SER | LEU | VAL | GLY | LYS | LEU | GLU | ||||
| 3 | THR | ASP | VAL | GLU | ILE | LYS | ALA | SER | ALA | ASP | ||||
| 4 | LYS | PHE | HIS | HIS | MET | PHE | ALA | GLY | LYS | PRO | ||||
| 5 | HIS | HIS | VAL | SER | LYS | ALA | SER | PRO | GLY | ASN | ||||
| 6 | ILE | GLN | GLY | CYS | ASP | LEU | HIS | GLU | GLY | ASP | ||||
| 7 | TRP | GLY | THR | VAL | GLY | SER | ILE | VAL | PHE | TRP | ||||
| 8 | ASN | TYR | VAL | HIS | ASP | GLY | GLU | ALA | LYS | VAL | ||||
| 9 | ALA | LYS | GLU | ARG | ILE | GLU | ALA | VAL | GLU | PRO | ||||
| 10 | ASP | LYS | ASN | LEU | ILE | THR | PHE | ARG | VAL | ILE | ||||
| 11 | GLU | GLY | ASP | LEU | MET | LYS | GLU | TYR | LYS | SER | ||||
| 12 | PHE | LEU | LEU | THR | ILE | GLN | VAL | THR | PRO | LYS | ||||
| 13 | PRO | GLY | GLY | PRO | GLY | SER | ILE | VAL | HIS | TRP | ||||
| 14 | HIS | LEU | GLU | TYR | GLU | LYS | ILE | SER | GLU | GLU | ||||
| 15 | VAL | ALA | HIS | PRO | GLU | THR | LEU | LEU | GLN | PHE | ||||
| 16 | CYS | VAL | GLU | VAL | SER | LYS | GLU | ILE | ASP | GLU | ||||
| 17 | HIS | LEU | LEU | ALA | GLU | GLU |
Samples:
sample: MLP-like protein 28, [U-13C; U-15N], 0.7 mM; bis-tris, [U-2H], 10 mM; Dithiothreitol, none, 5 mM; H2O, none, 95%; D2O, none, 5%
sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample | isotropic | sample_conditions_1 |
| 3D 13C-separated NOESY | sample | isotropic | sample_conditions_1 |
| 3D 13C-separated NOESY (AROMATIC) | sample | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v2004, Delagio,F. et al. - processing
XEASY v1.3, Eccles, C.; Guntert, P.; Billeter, M.; Wuthrich, K. - data analysis
SPSCAN v1.1.0, R.W. Glaser - data analysis
GARANT v2.1, C. Bartels - data analysis
CYANA v2.1, Guntert, P. -
X-PLOR NIH v2.9.3, SCHWIETERS, C.D., KUSZEWSKI, J.J., TJANDRA, N., CLORE, G.M. - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts