BMRB Entry 7241
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7241
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Title: Chromo 2 domain of cpSRP43 complexed with cpSRP54 peptide
Deposition date: 2006-07-24 Original release date: 2007-07-24
Authors: Kathir, Karuppanan; Vaithiyalingam, Sivaraja; Dakshinamurthy, Rajalingam; Henry, Ralph; Thallapuranam, Suresh Kumar
Citation: Kathir, Karuppanan; Vaithiyalingam, Sivaraja; Dakshinamurthy, Rajalingam; Henry, Ralph; Thallapuranam, Suresh Kumar. "Structural Characterization of the Interface of the Subunits Chloroplast Signal Recognition Particle (cpSRP) ? Determination of the Three-Dimensional Solution Structure of the Chromo Domain 2 ? cpSRP54 Peptide Complex" The BMRB entry is the only known published source for the data..
Assembly members:
CD2 of cpSRP43, polymer, 57 residues, 6539.1 Da.
cpSRP54 peptide, polymer, 14 residues, Formula weight is not available
Natural source: Common Name: Arabdopsis Taxonomy ID: 3702 Superkingdom: Eubacteria Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
CD2 of cpSRP43: GSQVFEYAEVDEIVEKRGKG
KDVEYLVRWKDGGDCEWVKG
VHVAEDVAKDYEDGLEY
cpSRP54 peptide: APPGTARRKRKADS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 151 |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 306 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD2 domain of cpSRP43 | 1 |
| 2 | cpSRP54 peptide | 2 |
Entities:
Entity 1, CD2 domain of cpSRP43 57 residues - 6539.1 Da.
| 1 | GLY | SER | GLN | VAL | PHE | GLU | TYR | ALA | GLU | VAL | ||||
| 2 | ASP | GLU | ILE | VAL | GLU | LYS | ARG | GLY | LYS | GLY | ||||
| 3 | LYS | ASP | VAL | GLU | TYR | LEU | VAL | ARG | TRP | LYS | ||||
| 4 | ASP | GLY | GLY | ASP | CYS | GLU | TRP | VAL | LYS | GLY | ||||
| 5 | VAL | HIS | VAL | ALA | GLU | ASP | VAL | ALA | LYS | ASP | ||||
| 6 | TYR | GLU | ASP | GLY | LEU | GLU | TYR |
Entity 2, cpSRP54 peptide 14 residues - Formula weight is not available
| 1 | ALA | PRO | PRO | GLY | THR | ALA | ARG | ARG | LYS | ARG | ||||
| 2 | LYS | ALA | ASP | SER |
Samples:
sample_1: CD2 domain of cpSRP43, [U-13C; U-15N], 1.0 ± 0.05 mM; cpSRP54 peptide 1.0 ± 0.05 mM; Phosphate 20 mM; NaCl 50 mM; sodium azide 1 mM
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | conditions_1 |
Software:
SPARKY -
NMR spectrometers:
- Bruker Avance 700 MHz
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