BMRB Entry 7230

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PDB ID: 2h49
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7230
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: The NMR Structure of an Internal Loop from 23S Ribosomal RNA Differs from its Structure in Crystals of the 50S Ribosomal Subunit PubMed: 17002278

Deposition date: 2006-07-14 Original release date: 2006-10-19

Authors: Shankar, N.

Citation: Shankar, Neelaabh; Kennedy, S.; Chen, G.; Krugh, T.; Turner, D.. "The NMR Structure of an Internal Loop from 23S Ribosomal RNA Differs from Its Structure in Crystals of 50S Ribosomal Subunits" Biochemistry 45, 11776-11789 (2006).

Assembly members:
5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3', polymer, 9 residues, Formula weight is not available
5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3', polymer, 9 residues, Formula weight is not available

Natural source: Common Name: Deinococcus radiodurans Taxonomy ID: 1299 Superkingdom: Eubacteria Kingdom: not available Genus/species: Deinococcus radiodurans

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3': GGCUAAGAC
5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3': GUCGAAGCC

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
- chemical_shift_set_2

Data type	Count
1H chemical shifts	102

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	5'-R(GPGPCPUPAPAPGPAP*C)-3'	1
2	5'-R(GPUPCPGPAPAPGPCP*C)-3'	2

Entities:

Entity 1, 5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3' 9 residues - Formula weight is not available

1

G

C

U

A

G

A

C

Entity 2, 5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3' 9 residues - Formula weight is not available

1

G

U

C

G

A

G

C

Samples:

sample_1: 5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3' 2 mM; 5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3' 2 mM; NaCl 80 mM; SODIUM PHOSPHATE 10 mM; Na2EDTA 0.5 mM; H2O 90%; D2O 10%

sample_2: 5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3' 2 mM; 5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3' 2 mM; NaCl 80 mM; SODIUM PHOSPHATE 10 mM; Na2EDTA 0.5 mM; D2O 100%

sample_cond_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
unknown	not available	isotropic	sample_cond_1

Software:

SPARKY v3.110 - data analysis

NMRDraw v2.3 - processing

DISCOVER v98 - refinement

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 500 MHz