BMRB Entry 7092
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7092
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Title: Chemical shifts of Vigna radiata Defensin 2 from Mung bean PubMed: 17444520
Deposition date: 2006-05-02 Original release date: 2007-05-04
Authors: Lin, Ku-Feng; Lyu, Ping-Chiang
Citation: Lin, Ku-Feng; Lee, Tian-Ren; Tsai, Ping-Hsing; Hsu, Ming-Pin; Chen, Ching-San; Lyu, Ping-Chiang. "Structure-based protein engineering for alpha-amylase inhibitory activity of plant defensin." Proteins 68, 530-540 (2007).
Assembly members:
VrD2, polymer, 47 residues, 5495 Da.
Natural source: Common Name: Mung bean Taxonomy ID: 157791 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Vigna radiata
Experimental source: Production method: Purified from the natural source
Entity Sequences (FASTA):
VrD2: KTCENLANTYRGPCFTTGSC
DDHCKNKEHLRSGRCRDDFR
CWCTRNC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 288 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VrD2 | 1 |
Entities:
Entity 1, VrD2 47 residues - 5495 Da.
| 1 | LYS | THR | CYS | GLU | ASN | LEU | ALA | ASN | THR | TYR | ||||
| 2 | ARG | GLY | PRO | CYS | PHE | THR | THR | GLY | SER | CYS | ||||
| 3 | ASP | ASP | HIS | CYS | LYS | ASN | LYS | GLU | HIS | LEU | ||||
| 4 | ARG | SER | GLY | ARG | CYS | ARG | ASP | ASP | PHE | ARG | ||||
| 5 | CYS | TRP | CYS | THR | ARG | ASN | CYS |
Samples:
sample_1: VrD2 2 ± 0.1 mM; Phosphate buffer 50 ± 1 mM; D2O 10%; H2O 90%
conditions_1: ionic strength: 50 mM; pH: 6.0; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H_2DNOESY | sample_1 | not available | conditions_1 |
| 1H_2DTOCSY | sample_1 | not available | conditions_1 |
| 1H_2DDQFCOSY | sample_1 | not available | conditions_1 |
Software:
SPARKY v3.111 -
NMR spectrometers:
- Bruker avance 600 MHz