BMRB Entry 7034
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7034
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C, and 15N chemical shift assignments for the zinc-finger region of human ZHX1 PubMed: 19348505
Deposition date: 2006-03-27 Original release date: 2006-04-05
Authors: Wienk, Hans; Lammers, Ivonne; Wu, Jin; Hotze, Anna; Wechselberger, Rainer; Kaptein, Rob; Folkers, Gert
Citation: Wienk, Hans; Lammers, Ivonne; Hotze, Anna; Wu, Jin; Wechselberger, Rainer; Owens, Ray; Stammers, David; Stuart, David; Kaptein, Robert; Folkers, Gert. "The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension." Biochemistry 48, 4431-4439 (2009).
Assembly members:
hZHX1-ZNF, polymer, 102 residues, 12110 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
hZHX1-ZNF: MAHHHHHHNQQNKKVEGGYE
CKYCTFQTPDLNMFTFHVDS
EHPNVVLNSSYVCVECNFLT
KRYDALSEHNLKYHPGEENF
KLTMVKRNNQTIFEQTINDL
TF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 309 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 532 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | polypeptide | 1 |
| 2 | zinc1 | 2 |
| 3 | zinc2 | 2 |
Entities:
Entity 1, polypeptide 102 residues - 12110 Da.
| 1 | MET | ALA | HIS | HIS | HIS | HIS | HIS | HIS | ASN | GLN | ||||
| 2 | GLN | ASN | LYS | LYS | VAL | GLU | GLY | GLY | TYR | GLU | ||||
| 3 | CYS | LYS | TYR | CYS | THR | PHE | GLN | THR | PRO | ASP | ||||
| 4 | LEU | ASN | MET | PHE | THR | PHE | HIS | VAL | ASP | SER | ||||
| 5 | GLU | HIS | PRO | ASN | VAL | VAL | LEU | ASN | SER | SER | ||||
| 6 | TYR | VAL | CYS | VAL | GLU | CYS | ASN | PHE | LEU | THR | ||||
| 7 | LYS | ARG | TYR | ASP | ALA | LEU | SER | GLU | HIS | ASN | ||||
| 8 | LEU | LYS | TYR | HIS | PRO | GLY | GLU | GLU | ASN | PHE | ||||
| 9 | LYS | LEU | THR | MET | VAL | LYS | ARG | ASN | ASN | GLN | ||||
| 10 | THR | ILE | PHE | GLU | GLN | THR | ILE | ASN | ASP | LEU | ||||
| 11 | THR | PHE |
Entity 2, zinc1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: hZHX1-ZNF, [U-98% 13C; U-99% 15N], 0.3 mM; NaPO4 30 mM; NaCl 100 mM; ZnCl2 50 microM; D2O 10%
conditions_1: pH: 8.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HN(CA)HA | sample_1 | not available | conditions_1 |
| CC(CO)NH | sample_1 | not available | conditions_1 |
| H(C)CH-TOCSY | sample_1 | not available | conditions_1 |
| (H)CCH-COSY | sample_1 | not available | conditions_1 |
| HNH-NOESY | sample_1 | not available | conditions_1 |
| HH-NOESY | sample_1 | not available | conditions_1 |
Software:
SPARKY v3, T. D. Goddard and D. G. Kneller - assignment
XWIN-NMR v3.5, BRUKER - processing
NMR spectrometers:
- Bruker Avance 700.13 MHz
- Bruker Avance 900.21 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts