BMRB Entry 6999
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6999
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Title: KorA PubMed: 16944275
Deposition date: 2006-02-21 Original release date: 2006-09-22
Authors: Rajasekar, Karthik; Bingle, Lewis; Thomas, Christopher; Hyde, Eva
Citation: Rajasekar, Karthik; Bingle, Lewis; Thomas, Christopher; Hyde, Eva. "1H, 13C and 15N assignments of the KorA global transcriptional repressor protein from the low copy number IncP-1 plasmid, RK2" J. Biomol. NMR 36, 71-71 (2006).
Assembly members:
KorA, polymer, 101 residues, 11303 Da.
Natural source: Common Name: Klebsiella aerogenes Taxonomy ID: 28451 Superkingdom: Bacteria Kingdom: not available Genus/species: Klebsiella aerogenes
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
KorA: MKKRLTESQFQEAIQGLEVG
QQTIEIARGVLVDGKPQATF
ATSLGLTRGAVSQAVHRVWA
AFEDKNLPEGYARVTAVLPE
HQAYIVRKWEADAKKKQETK
R
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 419 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 587 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KorA monomer, chain 1 | 1 |
| 2 | KorA monomer, chain 2 | 1 |
Entities:
Entity 1, KorA monomer, chain 1 101 residues - 11303 Da.
| 1 | MET | LYS | LYS | ARG | LEU | THR | GLU | SER | GLN | PHE | ||||
| 2 | GLN | GLU | ALA | ILE | GLN | GLY | LEU | GLU | VAL | GLY | ||||
| 3 | GLN | GLN | THR | ILE | GLU | ILE | ALA | ARG | GLY | VAL | ||||
| 4 | LEU | VAL | ASP | GLY | LYS | PRO | GLN | ALA | THR | PHE | ||||
| 5 | ALA | THR | SER | LEU | GLY | LEU | THR | ARG | GLY | ALA | ||||
| 6 | VAL | SER | GLN | ALA | VAL | HIS | ARG | VAL | TRP | ALA | ||||
| 7 | ALA | PHE | GLU | ASP | LYS | ASN | LEU | PRO | GLU | GLY | ||||
| 8 | TYR | ALA | ARG | VAL | THR | ALA | VAL | LEU | PRO | GLU | ||||
| 9 | HIS | GLN | ALA | TYR | ILE | VAL | ARG | LYS | TRP | GLU | ||||
| 10 | ALA | ASP | ALA | LYS | LYS | LYS | GLN | GLU | THR | LYS | ||||
| 11 | ARG |
Samples:
sample_1: KorA, [U-13C; U-15N], 1 mM; Phosphate Buffer 25 mM; EDTA 0.1 mM
conditions_1: pH: 6.2; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HN(CA)CO | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HB(CB)HA(CA)CONH | sample_1 | not available | conditions_1 |
| HB(CB)HA(CA)NH | sample_1 | not available | conditions_1 |
| HC(C)H-TOCSY | sample_1 | not available | conditions_1 |
| TOCSY-15N HSQC | sample_1 | not available | conditions_1 |
| (H)CC(CO)NH-TOCSY | sample_1 | not available | conditions_1 |
| H(CC)(CO)NH-TOCSY | sample_1 | not available | conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | not available | conditions_1 |
| (HB) CB (CG CD) HD | sample_1 | not available | conditions_1 |
| (HB) CB (CG CD CE) HE | sample_1 | not available | conditions_1 |
Software:
CCPN v1.0.8 -
NMRPipe -
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian Unity Plus 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAI47895 BAI47938 BAJ06625 |
| EMBL | CAA27596 CAG30914 CAK12746 |
| GB | AAK73380 AAO47408 AAO47415 AAO47422 AAO47428 |
| PIR | RGECKK |
| REF | WP_011205830 YP_006941392 YP_006941474 YP_006941560 YP_009065236 |
| SP | P03052 |
| TPE | CAJ85740 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts