BMRB Entry 6989
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6989
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Title: 1H, 13C, and 15N Chemical Shift Assignments for Ribosomal Protein S24E PubMed: 10476961
Deposition date: 2006-02-15 Original release date: 2007-03-06
Authors: Jeon, Byoung-Young; Hong, Enn-mi; Jung, Jin-won; Yee, Adelinda; Cheryl H, Arrowsmith; Lee, Weontae
Citation: Ban, N.; Nissen, P.; Hansen, J.; Capel, M.; Moore, Peter; Steitz, T.. "Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit." Nature 400, 841-847 (1999).
Assembly members:
Ribosomal Protein S24E, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: Thermoplasma acidophilum Taxonomy ID: 2303 Superkingdom: Archaea Kingdom: not available Genus/species: Thermoplasma acidophilum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Ribosomal Protein S24E: MDLIIKEKRDNPILKRKEIK
YVLKFDSSRTPSREEIKELI
AKHEGVDKELVIVDNNKQLT
GKHEIEGYTKIYADKPSAML
YEPDYELIRNGLKQKEAK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 404 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 603 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ribosomal Protein S24E | 1 |
Entities:
Entity 1, Ribosomal Protein S24E 98 residues - Formula weight is not available
| 1 | MET | ASP | LEU | ILE | ILE | LYS | GLU | LYS | ARG | ASP | ||||
| 2 | ASN | PRO | ILE | LEU | LYS | ARG | LYS | GLU | ILE | LYS | ||||
| 3 | TYR | VAL | LEU | LYS | PHE | ASP | SER | SER | ARG | THR | ||||
| 4 | PRO | SER | ARG | GLU | GLU | ILE | LYS | GLU | LEU | ILE | ||||
| 5 | ALA | LYS | HIS | GLU | GLY | VAL | ASP | LYS | GLU | LEU | ||||
| 6 | VAL | ILE | VAL | ASP | ASN | ASN | LYS | GLN | LEU | THR | ||||
| 7 | GLY | LYS | HIS | GLU | ILE | GLU | GLY | TYR | THR | LYS | ||||
| 8 | ILE | TYR | ALA | ASP | LYS | PRO | SER | ALA | MET | LEU | ||||
| 9 | TYR | GLU | PRO | ASP | TYR | GLU | LEU | ILE | ARG | ASN | ||||
| 10 | GLY | LEU | LYS | GLN | LYS | GLU | ALA | LYS |
Samples:
sample_1: Ribosomal Protein S24E, [U-13C; U-15N], 1.5 mM; photasuim phosphate 50 mM; NaN3 0.1 mM; H2O 90%; D2O 10%
sample_2: Ribosomal Protein S24E, [U-15N], 1.5 mM; photasuim phosphate 50 mM; NaN3 0.1 mM; H2O 90%; D2O 10%
sample_3: Ribosomal Protein S24E, [U-13C], 1.5 mM; photasuim phosphate 50 mM; NaN3 0.1 mM; D2O 100%
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_2 | not available | conditions_1 |
| HNCA | sample_2 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| H(CC)(CO)NH | sample_1 | not available | conditions_1 |
| HCCONH | sample_1 | not available | conditions_1 |
| 3D_15N-edited_NOESY | sample_2 | not available | conditions_1 |
| 3D_13C-edited_NOESY | sample_3 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian INOVA 500 MHz
- Bruker DRX 800 MHz
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts