BMRB Entry 6952
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6952
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Title: Violacin A PubMed: 16488428
Deposition date: 2006-01-27 Original release date: 2006-04-26
Authors: Ireland, D.; Craik, D.; Daly, N.
Citation: Ireland, D.; Colgrave, M.; Nguyencong, P.; Daly, N.; Craik, D.. "Discovery and Characterization of a Linear Cyclotide from Viola odorata: Implications for the Processing of Circular Proteins" J. Mol. Biol. 357, 1522-1535 (2006).
Assembly members:
Violacin A, polymer, 27 residues, Formula weight is not available
Natural source: Common Name: Viola odorata Taxonomy ID: 97441 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Viola odorata
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Violacin A: SAISCGETCFKFKCYTPRCS
CSYPVCK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Violacin A | 1 |
Entities:
Entity 1, Violacin A 27 residues - Formula weight is not available
| 1 | SER | ALA | ILE | SER | CYS | GLY | GLU | THR | CYS | PHE | ||||
| 2 | LYS | PHE | LYS | CYS | TYR | THR | PRO | ARG | CYS | SER | ||||
| 3 | CYS | SER | TYR | PRO | VAL | CYS | LYS |
Samples:
sample_1: Violacin A 1 mM; H2O 90%; D2O 10%
sample_2: Violacin A 1 mM; D2O 100%
sample_cond_1: pH: 3.8; pressure: 1 atm; temperature: 298 K
sample_cond_2: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| E-COSY | not available | not available | not available |
Software:
xwinnmr v3.5 - processing
DYANA v1.5 - refinement
CNS v1.1 - structure solution
NMR spectrometers:
- Bruker ARX 600 MHz