BMRB Entry 6920
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6920
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Mid1 BBox 1 PubMed: 16529770
Deposition date: 2005-12-08 Original release date: 2006-04-27
Authors: Massiah, Michael; Simmons, Brandi; Short, Keiran; Cox, Tim
Citation: Massiah, Michael; Simmons, Brandi; Short, Keiran; Cox, Tim. "Solution Structure of the RBCC/TRIM B-box1 Domain of Human MID1: B-box with a RING" J. Mol. Biol. 358, 532-545 (2006).
Assembly members:
B-box 1 type 1, polymer, 78 residues, 8620 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
B-box 1 type 1: QKASVSGPNSPSETRRERAF
DANTMTSAEKVLCQFCDQDP
AQDAVKTCVTCEVSYCDECL
KATHPNKKPFTGHRLIEP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 174 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 421 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Midline-1 B-box Type 1 | 1 |
| 2 | ZINC (II) ION, 1 | 2 |
| 3 | ZINC (II) ION, 2 | 2 |
Entities:
Entity 1, Midline-1 B-box Type 1 78 residues - 8620 Da.
Sequence corresponds to Gln87 to Pro164 in Midline1
| 1 | GLN | LYS | ALA | SER | VAL | SER | GLY | PRO | ASN | SER | ||||
| 2 | PRO | SER | GLU | THR | ARG | ARG | GLU | ARG | ALA | PHE | ||||
| 3 | ASP | ALA | ASN | THR | MET | THR | SER | ALA | GLU | LYS | ||||
| 4 | VAL | LEU | CYS | GLN | PHE | CYS | ASP | GLN | ASP | PRO | ||||
| 5 | ALA | GLN | ASP | ALA | VAL | LYS | THR | CYS | VAL | THR | ||||
| 6 | CYS | GLU | VAL | SER | TYR | CYS | ASP | GLU | CYS | LEU | ||||
| 7 | LYS | ALA | THR | HIS | PRO | ASN | LYS | LYS | PRO | PHE | ||||
| 8 | THR | GLY | HIS | ARG | LEU | ILE | GLU | PRO |
Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: B-box 1 type 1, [U-13C; U-15N], 0.75 ± 0.1 mM; ZINC (II) ION mM; Tris 50 mM; NaCl 300 mM
conditions_1: pH: 7.5; temperature: 294 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| HMQC-NOE-HSQC | sample_1 | not available | conditions_1 |
| 15N-HSQC-NOESY | sample_1 | not available | conditions_1 |
| 13C-HSQC-NOESY | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts