BMRB Entry 6885

Name: Assignment of 1H and 15N chemical shifts for the Ig1 module of FGFR1
Published: 2006-11-06

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PDB ID: 2ckn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6885
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Assignment of 1H and 15N chemical shifts for the Ig1 module of FGFR1 PubMed: 16609835

Deposition date: 2005-11-03 Original release date: 2006-11-06

Authors: Vladislav, Kiselyov; Vladimir, Berezin; Elisabeth, Bock; Flemming, Poulsen

Citation: Kiselyov, Vladislav; Berezin, Vladimir; Bock, Elisabeth; Poulsen, Flemming. "1H and 15N Resonance Assignment of the First Module of FGFR1" J. Biomol. NMR 36, 28-28 (2006).

Assembly members:
Ig1 module of FGFR1, polymer, 107 residues, Formula weight is not available

Natural source: Common Name: house mouse Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
Ig1 module of FGFR1: EARPAPTLPEQAQPWGVPVE VESLLVHPGDLLQLRCRLRD DVQSINWLRDGVQLVESNRT RITGEEVEVRDSIPADSGLY ACVTSSPSGSDTTYFSVNVS DALPSSE

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
15N chemical shifts	105
1H chemical shifts	595

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ig1 module	1

Entities:

Entity 1, Ig1 module 107 residues - Formula weight is not available

1

GLU

ALA

ARG

PRO

ALA

PRO

THR

LEU

PRO

GLU

2

GLN

ALA

GLN

PRO

TRP

GLY

VAL

PRO

VAL

GLU

3

VAL

GLU

SER

LEU

VAL

HIS

PRO

GLY

ASP

4

LEU

GLN

LEU

ARG

CYS

ARG

LEU

ARG

ASP

5

ASP

VAL

GLN

SER

ILE

ASN

TRP

LEU

ARG

ASP

6

GLY

VAL

GLN

LEU

VAL

GLU

SER

ASN

ARG

THR

7

ARG

ILE

THR

GLY

GLU

VAL

GLU

VAL

ARG

8

ASP

SER

ILE

PRO

ALA

ASP

SER

GLY

LEU

TYR

9

ALA

CYS

VAL

THR

SER

PRO

SER

GLY

SER

10

ASP

THR

TYR

PHE

SER

VAL

ASN

VAL

SER

11

ASP

ALA

LEU

PRO

SER

GLU

Samples:

sample_1: Ig1 module of FGFR1 2 mM; H2O; D2O; sodium phosphate 10 mM; NaCl 150 mM

sample_2: Ig1 module of FGFR1, [U-15N], 2 mM; H2O; D2O; sodium phosphate 10 mM; NaCl 150 mM

conditions_1: ionic strength: 150 mM; pH: 7.4; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
TOCSY	not available	not available	conditions_1
NOESY	not available	not available	conditions_1
15N-TOCSY-HSQC	not available	not available	conditions_1
15N-NOESY-HSQC	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Unity Inova 750 MHz
Varian Unity Inova 800 MHz

PDB	2CKN
DBJ	BAA02059
EMBL	CAA36175 CAA40932
GB	AAA37290 AAB19327 AAB19328 AAC52182 AAH10200
PRF	1908208A 2103285A
REF	NP_001073377 NP_034336 NP_077060 XP_001492195 XP_002925298
SP	P16092 Q04589

Biological Magnetic Resonance Data Bank