BMRB Entry 6769
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6769
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Title: Complete assignments of a hypothetical protein NE2066, from Nitrosomonas_europaea. Northeast Structural Genomics Consortium Target NeT1. PubMed: 16741959
Deposition date: 2005-08-10 Original release date: 2007-02-06
Authors: Srisailam, Sampath; Yee, Adelinda; Lukin, Jonathan; Semesi, Anthony; Arrowsmith, Cheryl
Citation: Srisailam, Sampath; Lukin, Jonathan; Yee, Adelinda; Semesi, Anthony; Arrowsmith, Cheryl. "Solution structure of acyl carrier protein from Nitrosomonas europaea." Proteins 64, 800-803 (2006).
Assembly members:
NE2066, polymer, 83 residues, 9169.3 Da.
Natural source: Common Name: Nitrosomonas europaea Taxonomy ID: 915 Superkingdom: Bacteria Kingdom: not available Genus/species: Nitrosomonas europaea
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
NE2066: MQHLEAVRNILGDVLNLGER
KHTLTASSVLLGNIPELDSM
AVVNVITALEEYFDFSVDDD
EISAQTFETLGSLALFVEHK
LSH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 348 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 577 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein | 1 |
Entities:
Entity 1, protein 83 residues - 9169.3 Da.
| 1 | MET | GLN | HIS | LEU | GLU | ALA | VAL | ARG | ASN | ILE | ||||
| 2 | LEU | GLY | ASP | VAL | LEU | ASN | LEU | GLY | GLU | ARG | ||||
| 3 | LYS | HIS | THR | LEU | THR | ALA | SER | SER | VAL | LEU | ||||
| 4 | LEU | GLY | ASN | ILE | PRO | GLU | LEU | ASP | SER | MET | ||||
| 5 | ALA | VAL | VAL | ASN | VAL | ILE | THR | ALA | LEU | GLU | ||||
| 6 | GLU | TYR | PHE | ASP | PHE | SER | VAL | ASP | ASP | ASP | ||||
| 7 | GLU | ILE | SER | ALA | GLN | THR | PHE | GLU | THR | LEU | ||||
| 8 | GLY | SER | LEU | ALA | LEU | PHE | VAL | GLU | HIS | LYS | ||||
| 9 | LEU | SER | HIS |
Samples:
sample_1: protein, [U-15N; U-13C], 1 ± 0.2 mM; MOPS 10 ± 0.1 mM; NaCl 450 ± 0.1 mM; DTT 10 ± 0.1 mM; Zinc Sulfate 10 ± 0.1 uM; Benzamidine 1 ± 0.1 mM; Sodium azide 0.01 ± 0.1 %; D2O 5 ± 1 %
conditions_1: ionic strength: 0.45 M; pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HNcaCO | sample_1 | not available | conditions_1 |
| CBCAcoNH | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HNcoCA | sample_1 | not available | conditions_1 |
| HBHAcoNH | sample_1 | not available | conditions_1 |
| CCTOCSY_NH | sample_1 | not available | conditions_1 |
| HCCTOCSY_NH | sample_1 | not available | conditions_1 |
| 13C HSQC | sample_1 | not available | conditions_1 |
| HCCHCOSY | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| 15N NOESY | sample_1 | not available | conditions_1 |
| 13C NOESY | sample_1 | not available | conditions_1 |
| HNCB | sample_1 | not available | conditions_1 |
Software:
SPARKY v3.106 -
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
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