BMRB Entry 6746
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6746
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Title: Assignment of phnA-like protein rp4479
Deposition date: 2005-07-25 Original release date: 2006-02-23
Authors: Wu, Bin; Yee, Adelina; Ramelot, Theresa; Semesi, Anthony; Lemak, Alexander; Kennedy, Michael; Arrowsmith, Cheryl
Citation: Wu, Bin; Yee, Adelina; Ramelot, Theresa; Semesi, Anthony; Lemak, Alexander; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of phnA-like protein rp4479 from Rhodopseudomonas palustris" The BMRB entry is the only known published source for the data..
Assembly members:
phnA-like protein rp4479, polymer, 74 residues, 7933 Da.
Natural source: Common Name: Rhodopseudomonas palustris Taxonomy ID: 2303 Superkingdom: Bacteria Kingdom: not available Genus/species: rhodopseudomonas palustris
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
phnA-like protein rp4479: MSIEVRDCNGALLADGDNVS
LIKDLKLKGSSTVLKRGTMI
RGIRLTDSEDEIEGRTDKIK
GLVLRTEFLKKAGS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 520 |
| 13C chemical shifts | 313 |
| 15N chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rp4479 | 1 |
Entities:
Entity 1, rp4479 74 residues - 7933 Da.
| 1 | MET | SER | ILE | GLU | VAL | ARG | ASP | CYS | ASN | GLY | ||||
| 2 | ALA | LEU | LEU | ALA | ASP | GLY | ASP | ASN | VAL | SER | ||||
| 3 | LEU | ILE | LYS | ASP | LEU | LYS | LEU | LYS | GLY | SER | ||||
| 4 | SER | THR | VAL | LEU | LYS | ARG | GLY | THR | MET | ILE | ||||
| 5 | ARG | GLY | ILE | ARG | LEU | THR | ASP | SER | GLU | ASP | ||||
| 6 | GLU | ILE | GLU | GLY | ARG | THR | ASP | LYS | ILE | LYS | ||||
| 7 | GLY | LEU | VAL | LEU | ARG | THR | GLU | PHE | LEU | LYS | ||||
| 8 | LYS | ALA | GLY | SER |
Samples:
sample_1: phnA-like protein rp4479, [U-13C; U-15N], 1 mM; NaCl 450 mM; Phosphate 25 mM
cond_set1: pH: 6.5; temperature: 298 K; ionic strength: 0.45 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCAcoNH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CCTOCSY_NH | not available | not available | not available |
| HCCTOCSY_NH | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HCCHTOCSY | not available | not available | not available |
| 15N NOESY | not available | not available | not available |
| 13C NOESY | not available | not available | not available |
| 13,13C 4d CCNOESY | not available | not available | not available |
Software:
SPARKY v3.95 - spectral analysis, assignemt
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAE29941 |
| GB | ACF03482 ADU46252 |
| REF | NP_949835 WP_011160033 YP_001993957 YP_004110985 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts