BMRB Entry 6741
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6741
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Title: NMR Structure of Mini-B, an N-terminal - C-terminal construct from Surfactant Protein B (SP-B), in Sodium Dodecyl Sulfate (SDS) and Hexafluoroisopropanol (HFIP) PubMed: 17845058
Deposition date: 2005-07-21 Original release date: 2007-11-14
Authors: Booth, V.; Sarker, M.; Keough, K.; Waring, A.; Walther, F.
Citation: Sarker, M.; Waring, A.; Walther, F.; Keough, K.; Booth, V.. "Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles" Biochemistry 46, 11047-11056 (2007).
Assembly members:
Pulmonary surfactant-associated protein B, polymer, 34 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Pulmonary surfactant-associated protein B: CWLCRALIKRIQAMIPKGGR
MLPQLVCRLVLRCS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 554 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pulmonary surfactant-associated protein B | 1 |
Entities:
Entity 1, Pulmonary surfactant-associated protein B 34 residues - Formula weight is not available
| 1 | CYS | TRP | LEU | CYS | ARG | ALA | LEU | ILE | LYS | ARG | ||||
| 2 | ILE | GLN | ALA | MET | ILE | PRO | LYS | GLY | GLY | ARG | ||||
| 3 | MET | LEU | PRO | GLN | LEU | VAL | CYS | ARG | LEU | VAL | ||||
| 4 | LEU | ARG | CYS | SER |
Samples:
sample_1: Pulmonary surfactant-associated protein B, [15N]-Leu(3,7,22,25,29,31), Ala(6,13), and Gly(18), 1.5 mM; SDS 150 mM; DSS 0.4 mM; NaN3 0.2 mM; H2O 90%; D2O 10%
sample_2: Pulmonary surfactant-associated protein B, [15N]-Leu(3,7,22,25,29,31), Ala(6,13), and Gly(18), 1.5 mM; HFIP 40 mM; DSS 0.4 mM; NaN3 0.2 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 5; temperature: 318 K; ionic strength: 0 M; pressure: 1 atm
sample_cond_2: pH: 5; temperature: 288 K; ionic strength: 0 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 15N-separated TOCSY | not available | not available | not available |
Software:
NMRPipe v2.2 - processing
Sparky v3.110 - data analysis
CNS v1.1 - refinement
MOLMOL v2K.2 - data analysis
NMR spectrometers:
- Bruker AVANCE 500 MHz
Related Database Links:
| PDB |