BMRB Entry 6728
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6728
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Title: NMR STRUCTURAL STUDIES OF A POTASSIUM CHANNEL/CHARYBDOTOXIN COMPLEX PubMed: 16313186
Deposition date: 2005-07-08 Original release date: 2006-08-15
Authors: Yu, Liping; Olejniczak, Edward
Citation: Yu, Liping; Sun, Chaohong; Song, Danying; Shen, Jianwei; Xu, Nan; Gunasekera, Angelo; Haiduk, Philip; Olejniczak, Edward. "Nuclear Magnetic Resonance Structural Studies of a Potassium Channel-Charybdotoxin Complex" Biochemistry 44, 15834-15841 (2005).
Assembly members:
potassium channel, polymer, 129 residues, Formula weight is not available
Charybdotoxin, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: Streptomyces lividans Taxonomy ID: 1916 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces lividans
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
potassium channel: MPPMLSGLLARLVKLLLGRH
GSALHWRAAGAATVLLVIVL
LAGSYLAVLAERGAPGAALI
SYPDALWWSVETATTVGYGD
LYPVTLWGRCVAVVVMVAGI
TSYGLVFAAVATWFVGREQE
RRGHFVRHS
Charybdotoxin: XFTNVSCTTSKECWSVCQRL
HNTSRGKCMNKKCRCYS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 46 |
| 1H chemical shifts | 142 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | potassium channel KcsA, chain 1 | 1 |
| 2 | potassium channel KcsA, chain 2 | 1 |
| 3 | potassium channel KcsA, chain 3 | 1 |
| 4 | potassium channel KcsA, chain 4 | 1 |
| 5 | Charybdotoxin | 2 |
Entities:
Entity 1, potassium channel KcsA, chain 1 129 residues - Formula weight is not available
| 1 | MET | PRO | PRO | MET | LEU | SER | GLY | LEU | LEU | ALA | ||||
| 2 | ARG | LEU | VAL | LYS | LEU | LEU | LEU | GLY | ARG | HIS | ||||
| 3 | GLY | SER | ALA | LEU | HIS | TRP | ARG | ALA | ALA | GLY | ||||
| 4 | ALA | ALA | THR | VAL | LEU | LEU | VAL | ILE | VAL | LEU | ||||
| 5 | LEU | ALA | GLY | SER | TYR | LEU | ALA | VAL | LEU | ALA | ||||
| 6 | GLU | ARG | GLY | ALA | PRO | GLY | ALA | ALA | LEU | ILE | ||||
| 7 | SER | TYR | PRO | ASP | ALA | LEU | TRP | TRP | SER | VAL | ||||
| 8 | GLU | THR | ALA | THR | THR | VAL | GLY | TYR | GLY | ASP | ||||
| 9 | LEU | TYR | PRO | VAL | THR | LEU | TRP | GLY | ARG | CYS | ||||
| 10 | VAL | ALA | VAL | VAL | VAL | MET | VAL | ALA | GLY | ILE | ||||
| 11 | THR | SER | TYR | GLY | LEU | VAL | PHE | ALA | ALA | VAL | ||||
| 12 | ALA | THR | TRP | PHE | VAL | GLY | ARG | GLU | GLN | GLU | ||||
| 13 | ARG | ARG | GLY | HIS | PHE | VAL | ARG | HIS | SER |
Entity 2, Charybdotoxin 37 residues - Formula weight is not available
| 1 | PCA | PHE | THR | ASN | VAL | SER | CYS | THR | THR | SER | ||||
| 2 | LYS | GLU | CYS | TRP | SER | VAL | CYS | GLN | ARG | LEU | ||||
| 3 | HIS | ASN | THR | SER | ARG | GLY | LYS | CYS | MET | ASN | ||||
| 4 | LYS | LYS | CYS | ARG | CYS | TYR | SER |
Samples:
sample_1: potassium channel0.030 – 1.0 mM; Charybdotoxin mM
experimental_conditions: pH: 7.5; temperature: 315 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 13C-edited NOESY | sample_1 | not available | experimental_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz