BMRB Entry 6715

Name: Solution NMR Structure of the Membrane Protein Stannin
Published: 2007-01-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6715

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution NMR Structure of the Membrane Protein Stannin PubMed: 16246365

Deposition date: 2005-06-30 Original release date: 2007-01-29

Authors: Buck-Koehntop, B.; Mascioni, A.; Buffy, J.; Veglia, G.

Citation: Buck-Koehntop, B.; Mascioni, A.; Buffy, J.; Veglia, G.. "Structure, Dynamics, and Membrane Topology of Stannin: A Mediator of Neuronal Cell Apoptosis Induced by Trimethyltin Chloride." J. Mol. Biol. 354, 652-665 (2005).

Assembly members:
Stannin, polymer, 90 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
Stannin: GSMSIMDHSPTTGVVTVIVI LIAIAALGALILGCWCYLRL QRISQSEDEESIVGDGETKE PFLLVQYSAKGPCVERKAKL MTPNGPEVHG

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	165
13C chemical shifts	170
15N chemical shifts	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Stannin	1

Entities:

Entity 1, Stannin 90 residues - Formula weight is not available

1	GLY	SER	MET	SER	ILE	MET	ASP	HIS	SER	PRO
2	THR	THR	GLY	VAL	VAL	THR	VAL	ILE	VAL	ILE
3	LEU	ILE	ALA	ILE	ALA	ALA	LEU	GLY	ALA	LEU
4	ILE	LEU	GLY	CYS	TRP	CYS	TYR	LEU	ARG	LEU
5	GLN	ARG	ILE	SER	GLN	SER	GLU	ASP	GLU	GLU
6	SER	ILE	VAL	GLY	ASP	GLY	GLU	THR	LYS	GLU
7	PRO	PHE	LEU	LEU	VAL	GLN	TYR	SER	ALA	LYS
8	GLY	PRO	CYS	VAL	GLU	ARG	LYS	ALA	LYS	LEU
9	MET	THR	PRO	ASN	GLY	PRO	GLU	VAL	HIS	GLY

Samples:

sample_1: Stannin, [U-15N], 0.5 – 0.7 mM; phosphate buffer 20 mM; d25-sodium dodecyl sulfate 600 mM; sodium azide 0.02%; DTT 0.7 mM; H2O 90%; D2O 10%

sample_2: Stannin, [U-13C; U-15N], 0.5 – 0.7 mM; phosphate buffer 20 mM; d25-sodium dodecyl sulfate 600 mM; sodium azide 0.02%; DTT 0.7 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.0; temperature: 333 K; ionic strength: 120 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available

Software:

VNMR v6.1c - collection

NMRPipe v97.027.12.56 - processing

Sparky v3.111 - data analysis

X-PLOR v2.11 - structure solution, refinement

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1ZZA
DBJ	BAE29024 BAI46912
EMBL	CAB82314 CAG46924 CAG46935 CAL37610
GB	AAC28427 AAC28428 AAC83231 AAH06961 AAH17685
REF	NP_001029255 NP_001107194 NP_001180368 NP_003489 NP_033249
SP	O75324 P61807 P61808 Q17Q87
TPG	DAA15615

Biological Magnetic Resonance Data Bank