BMRB Entry 6478
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6478
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Title: The N-domain of the transcription factor AbrB PubMed: 15939023
Deposition date: 2005-02-02 Original release date: 2005-06-12
Authors: Coles, Murray; Truffault, Vincent; Lupas, Andrei
Citation: Coles, Murray; Djuranovic, Sergej; Soding, Johannes; Frickey, Tancred; Koretke, Kristin; Truffault, Vincent; Martin, Joerg; Lupas, Andrei. "AbrB-like Transcription Factors Assume a Swapped Hairpin Fold that Is Evolutionarily Related to Double-Psi beta Barrels" Structure (Camb) 13, 919-928 (2005).
Assembly members:
Transition state regulatory protein abrB, polymer, 53 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Esherichia coli
Entity Sequences (FASTA):
Transition state regulatory protein abrB: MFMKSTGIVRKVDELGRVVI
PIELRRTLGIAEKDALEIYV
DDEKIILKKYKPN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 406 |
| 13C chemical shifts | 222 |
| 15N chemical shifts | 55 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Transition state regulatory protein abrB, chain A | 1 |
| 2 | Transition state regulatory protein abrB, chain B | 1 |
Entities:
Entity 1, Transition state regulatory protein abrB, chain A 53 residues - Formula weight is not available
| 1 | MET | PHE | MET | LYS | SER | THR | GLY | ILE | VAL | ARG | ||||
| 2 | LYS | VAL | ASP | GLU | LEU | GLY | ARG | VAL | VAL | ILE | ||||
| 3 | PRO | ILE | GLU | LEU | ARG | ARG | THR | LEU | GLY | ILE | ||||
| 4 | ALA | GLU | LYS | ASP | ALA | LEU | GLU | ILE | TYR | VAL | ||||
| 5 | ASP | ASP | GLU | LYS | ILE | ILE | LEU | LYS | LYS | TYR | ||||
| 6 | LYS | PRO | ASN |
Samples:
sample_1: Transition state regulatory protein abrB, [U-15N], 0.5 mM; KPO4 20.0 mM; KCl 50.0 mM; NaN3 0.02%
sample_2: Transition state regulatory protein abrB, [U-15N], 0.25 mM; Transition state regulatory protein abrB, [U-13C], 0.25 mM; KPO4 20.0 mM; KCl 50.0 mM; NaN3 0.02%
sample_cond_1: pH: 5.8; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
XWINNMR v3.5 - collection, processing
NMRview - data analysis
X-PLOR v2.9.7 - structure solution, refinement
NMR spectrometers:
- Bruker DMX 900 MHz
Related Database Links:
| BMRB | 17650 4281 |
| PDB | |
| DBJ | BAA05272 BAI83483 BAL15827 BAM48968 BAM56238 |
| EMBL | CAA31307 CAA43955 CAB11813 CBI41163 CCF03572 |
| GB | AAA22195 AAP07140 AAP24090 AAS38971 AAT29114 |
| PRF | 1715209A |
| REF | NP_387918 NP_829939 NP_842604 NP_976363 WP_000843034 |
| SP | P08874 |
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