BMRB Entry 6448
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6448
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Title: Solution structure of the hypothetical protein ytfP from Escherichia coli. Northeast Structural Genomics target ER111.
Deposition date: 2004-12-28 Original release date: 2005-06-12
Authors: Aramini, J.; Huang, Y.; Swapna, G.; Paranji, R.; Xiao, R.; Shastry, R.; Acton, T.; Cort, J.; Kennedy, M.; Montelione, G.
Citation: Aramini, J.; Swapna, G.; Huang, Y.; Rajan, P.; Xiao, R.; Shastry, R.; Acton, T.; Cort, J.; Kennedy, M.; Montelione, G.. "1H, 13C, and 15N Resonance Assignments for Escherichia coli ytfP, a Member of the Broadly Conserved UPF0131 Protein Domain Family" J. Biomol. NMR 33, 197-197 (2005).
Assembly members:
Hypothetical UPF0131 protein ytfP, polymer, 121 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Hypothetical UPF0131 protein ytfP: MRIFVYGSLRHKQGNSHWMT
NAQLLGDFSIDNYQLYSLGH
YPGAVPGNGTVHGEVYRIDN
ATLAELDALRTRGGEYARQL
IQTPYGSAWMYVYQRPVDGL
KLIESGDWLDRDKLEHHHHH
H
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 797 |
| 13C chemical shifts | 508 |
| 15N chemical shifts | 127 |
| coupling constants | 61 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Hypothetical UPF0131 protein ytfP | 1 |
Entities:
Entity 1, Hypothetical UPF0131 protein ytfP 121 residues - Formula weight is not available
| 1 | MET | ARG | ILE | PHE | VAL | TYR | GLY | SER | LEU | ARG | ||||
| 2 | HIS | LYS | GLN | GLY | ASN | SER | HIS | TRP | MET | THR | ||||
| 3 | ASN | ALA | GLN | LEU | LEU | GLY | ASP | PHE | SER | ILE | ||||
| 4 | ASP | ASN | TYR | GLN | LEU | TYR | SER | LEU | GLY | HIS | ||||
| 5 | TYR | PRO | GLY | ALA | VAL | PRO | GLY | ASN | GLY | THR | ||||
| 6 | VAL | HIS | GLY | GLU | VAL | TYR | ARG | ILE | ASP | ASN | ||||
| 7 | ALA | THR | LEU | ALA | GLU | LEU | ASP | ALA | LEU | ARG | ||||
| 8 | THR | ARG | GLY | GLY | GLU | TYR | ALA | ARG | GLN | LEU | ||||
| 9 | ILE | GLN | THR | PRO | TYR | GLY | SER | ALA | TRP | MET | ||||
| 10 | TYR | VAL | TYR | GLN | ARG | PRO | VAL | ASP | GLY | LEU | ||||
| 11 | LYS | LEU | ILE | GLU | SER | GLY | ASP | TRP | LEU | ASP | ||||
| 12 | ARG | ASP | LYS | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
| 13 | HIS |
Samples:
sample_1: Hypothetical UPF0131 protein ytfP, [U-13C; U-15N], 1.3 mM; NH4OAC 20 mM; CACL2 5 mM; NaCL 0.1 M; DTT 10 mM; NAN3 0.02%; D2O 5%; H2O 95%
sample_2: Hypothetical UPF0131 protein ytfP, [U-13C; U-15N], 1.3 mM; NH4OAC 20 mM; CACL2 5 mM; NaCL 0.1 M; DTT 10 mM; NAN3 0.02%; D2O 100%
sample_3: Hypothetical UPF0131 protein ytfP, [U-5% 13C; U-15N], 1.1 mM; NH4OAC 20 mM; CACL2 5 mM; NaCL 0.1 M; DTT 10 mM; NAN3 0.02%; D2O 5%; H2O 95%
sample_cond_1: pH: 5.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-SEPARATED NOESY | not available | not available | not available |
| 3D 13C-SEPARATED NOESY (aliph) | not available | not available | not available |
| 4D 13C-SEPARATED NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HIGH RESOLUTION CH-HSQC | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
| HCCH COSY | not available | not available | not available |
| 3D 13C-SEPARATED NOESY (arom) | not available | not available | not available |
| 3D HNCOCACB | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D HNCOCA | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCACO | not available | not available | not available |
| HCCONH | not available | not available | not available |
| CCONH | not available | not available | not available |
| HACANH | not available | not available | not available |
| HACACONH | not available | not available | not available |
| HBCBcgcdHD | not available | not available | not available |
Software:
AUTOSTRUCTURE 2.1.0, XPLOR-NIH 2.0.6, CNS v1.1 - refinement
VNMR v6.1C - structure solution
PSVS v1.0 - structure solution
NMRPIPE v2.3 - structure solution
SPARKY v3.91 - structure solution
AUTOASSIGN v1.14 - structure solution
HYPER v3.2 - structure solution
PDBSTAT v3.27 - structure solution
NMR spectrometers:
- Varian Inova 800 MHz
- Varian Inova 750 MHz
- Varian Inova 600 MHz
- Varian Inova 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAB38623 BAE78223 BAG80052 BAI28527 BAI33701 |
| EMBL | CAP78743 CAQ34574 CAQ91721 CAR01197 CAR05964 |
| GB | AAA97119 AAC77179 AAG59420 AAN45683 AAN83741 |
| REF | NP_290854 NP_313227 NP_418643 NP_709976 NP_757167 |
| SP | P0AE48 P0AE49 P0AE50 P0AE51 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts